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NHX1 and eIF4A1-stacked transgenic sweetpotato shows enhanced tolerance to drought stress

  • Yandi Zhang
  • Gaifang Deng
  • Weijuan Fan
  • Ling Yuan
  • Hongxia WangEmail author
  • Peng ZhangEmail author
Original Article

Abstract

Key message

Co-expression of Na+/H+ antiporter NHX1 and DEAD-box RNA helicase eIF4A1 from Arabidopsis positively regulates drought stress tolerance by improving ROS scavenging capacity and maintaining membrane integrity in sweetpotato.

Abstract

Plants evolve multiple strategies for stress adaptation in nature. To improve sweetpotato resistance to drought stress, transgenic sweetpotato plants overexpressing the Arabidopsis Na+/H+ antiporter, NHX1, and the translation initiation factor elF4A1 were characterized for phenotypic traits and physiological performance. Without drought treatment, the NHX1elF4A1 stacked lines (NE lines) showed normal, vigorous growth comparable to the WT plants. The NE plants showed dense green foliage with delayed leaf senescence and developed more roots than WT plants under drought treatment for 18 days. Compared to WT plants, higher level of reactive oxygen scavenging capacity was detected in NE lines as indicated by reduced H2O2 accumulation as well as increased superoxide dismutase activity and proline content. The relative ion leakage and malondialdehyde content were reduced in NE plants, indicating improved maintenance of intact membranes system. Both NE plants and NHX1-overexpressing plants (N lines) showed larger aerial parts and well-developed root system compared to WT plants under the drought stress conditions, likely due to the improved antioxidant capacity. The NE plants showed better ROS scavenging than N-line plants. All N- and NE-line plants produced normal storage roots with similar yields as WT in the field under normal growth conditions. These results demonstrated the potential to enhance sweetpotato productivity through stacking genes that are involved in ion compartmentalization and translation initiation.

Keywords

Sweetpotato Drought stress NHX1 eIF4A1 Gene-stacking Overexpression Physiological parameter Phenotype 

Abbreviations

OE

Overexpression

ROS

Reactive oxygen species

qRT-PCR

Quantitative reverse transcription-polymerase chain reaction

Notes

Acknowledgements

This work was supported by the grants from the National Key R&D Program of China (2018YFD1000700, 2018YFD1000705), the National Natural Science Foundation of China (31771854) and China Scholarship Council (201804910055). We thank Prof. Sitakanta Pattanaik from UK for corrections the manuscript.

Author contribution statement

YZ analyzed the data and drafted the manuscript. GD performed the majority of the experiments. WF generated the NE transgenic lines. LY revised the manuscript and provided helpful suggestions. HW and PZ coordinated and designed the study and revised the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

299_2019_2454_MOESM1_ESM.docx (370 kb)
Supplementary material 1 (DOCX 370 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological SciencesChinese Academy of ScienceShanghaiChina
  2. 2.University of Chinese Academy of SciencesBeijingChina
  3. 3.Shanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Plant Science Research CenterChinese Academy of ScienceShanghaiChina
  4. 4.Department of Plant and Soil Sciences and Kentucky Tobacco Research and Development CenterUniversity of KentuckyLexingtonUSA

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