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Agrobacterium-mediated transient transformation of Bienertia sinuspersici to assay recombinant protein distribution between dimorphic chloroplasts

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Abstract

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Bienertia sinuspersici, a species capable of performing C4 photosynthesis within individual photosynthetic cells, can be transformed with Agrobacterium allowing for the analysis of subcellular protein distribution under preservation of the unique cellular morphology.

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Acknowledgements

Work in the S.O. lab was supported by the Deutsche Forschungsgemeinschaft (OF 106/1-1) and S.O. and I.H were funded by the “Cooperative Research Program for Agriculture Science & Technology Development (Project No. 0109532016)”, Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. Institute for Botany, Leibniz University Hannover, Herrenhaeuser Strasse 2, 30419, Hannover, Germany

    Diana Wimmer, Philipp Bohnhorst & Sascha Offermann

  2. Genebank Department, Cryo and Stress Biology Group, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK-Gatersleben), Corrensstrasse 3, 06466, Stadt Seeland, Germany

    Daniela Impe

  3. Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Pohang, 790–784, South Korea

    Inhwan Hwang

Authors
  1. Diana Wimmer
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  2. Philipp Bohnhorst
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  3. Daniela Impe
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  4. Inhwan Hwang
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  5. Sascha Offermann
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Correspondence to Sascha Offermann.

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Communicated by Neal Stewart.

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Wimmer, D., Bohnhorst, P., Impe, D. et al. Agrobacterium-mediated transient transformation of Bienertia sinuspersici to assay recombinant protein distribution between dimorphic chloroplasts. Plant Cell Rep 38, 779–782 (2019). https://doi.org/10.1007/s00299-019-02375-4

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  • DOI: https://doi.org/10.1007/s00299-019-02375-4

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