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The metal-binding domain of wheat heavy metal ATPase 2 (TaHMA2) is involved in zinc/cadmium tolerance and translocation in Arabidopsis

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Cysteine in the N-terminal metal-binding domain (N-MBD) of TaHMA2 participates in Zn2+/Cd2+ binding and translocation in Arabidopsis.

Abstract

Wheat heavy metal ATPase 2 (TaHMA2) can transport Zn2+ and Cd2+ across membranes. A previous study showed that cysteine (Cys) and glutamate residues in the N-terminal metal-binding domain (N-MBD) were necessary for metal-binding and translocation of TaHMA2 in yeast. However, the function of TaHMA2 in plants was not fully revealed. In this study, we investigated the roles of the CCxxE and CPC motifs in the N-MBD and the N/C-terminal regions of TaHMA2 in Zn2+/Cd2+ translocation in root and shoot of Arabidopsis. Compared with the wild type, overexpression of TaHMA2 and the TaHMA2 derivative (glutamic substituted for alanine from CCxxE) in Arabidopsis increased root length, fresh weight and enhanced Zn2+/Cd2+ root-to-shoot translocation. The plants with a truncated N/C-terminal of TaHMA2 were impaired in Zn2+/Cd2+ tolerance and translocation, while mutagenesis of Cys in the N-MBD reduced the tolerance and transport activity of TaHMA2, suggesting the involvement of Cys in Zn2+/Cd2+ binding and translocation in Arabidopsis. This study therefore provides a theoretical possibility for the application of TaHMA2 in transgenic breeding to regulate metal element balance in crop plants.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant No. U1632111, Grant No. 61672489), and the Southeast Asia Biodiversity Research Institute, Chinese Academy of Sciences (Grant No. Y4ZK111B01), and the Chinese Academy of Sciences (Grant No. KJRH2015-001).

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Author notes

  1. Kun Qiao and Liang Gong contributed equally to this work.

Authors and Affiliations

  1. College of Life Science, University of the Chinese Academy of Sciences, Beijing, China

    Kun Qiao, Liang Gong, Hong Wang & Tuanyao Chai

  2. Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, Guangdong Engineering Research Center for Marine Algal Biotechnology, College of Life Science and Oceanography, Shenzhen University, Shenzhen, China

    Kun Qiao

  3. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China

    Yanbao Tian & Tuanyao Chai

  4. The Innovative Academy of Seed Design (INASEED), Chinese Academy of Sciences, Beijing, China

    Tuanyao Chai

  5. Southeast Asia Biodiversity Research Institute, Chinese Academy of Science, Yezin, Nay Pyi Taw, 05282, Myanmar

    Tuanyao Chai

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  1. Kun Qiao
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  2. Liang Gong
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  3. Yanbao Tian
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  4. Hong Wang
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Correspondence to Hong Wang or Tuanyao Chai.

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Communicated by Kang Chong.

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Qiao, K., Gong, L., Tian, Y. et al. The metal-binding domain of wheat heavy metal ATPase 2 (TaHMA2) is involved in zinc/cadmium tolerance and translocation in Arabidopsis. Plant Cell Rep 37, 1343–1352 (2018). https://doi.org/10.1007/s00299-018-2316-3

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