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Engineering herbicide-resistant watermelon variety through CRISPR/Cas9-mediated base-editing

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Fig. 1


  • Chen Y, Wang Z, Ni H, Xu Y, Chen Q, Jiang L (2017) CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci 60:520–523

    Article  PubMed  CAS  Google Scholar 

  • Grey TL, Bridges DC, Nesmith DS (2000) Tolerance of cucurbits to the herbicides clomazone, ethalfluralin and pendimethalin. II Watermelon Hortscience 35:637–641

    CAS  Google Scholar 

  • Huang S, Weigel D, Beachy RN, Li J (2016) A proposed regulatory framework for genome-edited crops. Nat Genet 48:109–111

    Article  PubMed  CAS  Google Scholar 

  • Komor AC, Kim YB, Packer MS, Zuris JA, Liu DR (2016) Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature 533:420–424

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Li Z, Liu ZB, Xing A, Moon BP, Koellhoffer JP, Huang L, Ward RT, Clifton E, Falco SC, Cigan AM (2015) Cas9-guide RNA directed genome editing in soybean. Plant Physiol 169:960–970

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Ren B, Yan F, Kuang Y, Li N, Zhang D, Lin H, Zhou H (2017) A CRISPR/Cas9 toolkit for efficient targeted base editing to induce genetic variations in rice. Sci China Life Sci 60:516–519

    Article  PubMed  Google Scholar 

  • Shimatani Z, Kashojiya S, Takayama M, Terada R, Arazoe T, Ishii H, Teramura H, Yamamoto T, Komatsu H, Miura K (2017) Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion. Nat Biotechnol 35:441–443

    Article  PubMed  CAS  Google Scholar 

  • Svitashev S, Young JK, Schwartz C, Gao H, Falco SC, Cigan AM (2015) Targeted mutagenesis, precise gene editing, and site-specific gene insertion in maize using Cas9 and guide RNA. Plant Physiol 169:931–945

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Tian S, Jiang L, Gao Q, Zhang J, Zong M, Zhang H, Ren Y, Guo S, Gong G, Liu F, Xu Y (2017) Efficient CRISPR/Cas9-based gene knockout in watermelon. Plant Cell Rep 36:399–406

    Article  PubMed  CAS  Google Scholar 

  • Yu Q, Powles SB (2014) Resistance to AHAS inhibitor herbicides: current understanding. Pest Manag Sci 70:1340–1350

    Article  PubMed  CAS  Google Scholar 

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This work was supported by grants from the National Key R&D Program of China (2018YFD010062), Beijing science & technology program (D171100007617001), Beijing Academy of Agricultural and Forestry Sciences (KJCX20180427), National Natural Science Foundation of China (31701943, 31471785), the Ministry of Agriculture of China (CARS-26), Beijing Scholar Program (BSP026), BaGui Scholar Program.

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Correspondence to Linjian Jiang or Yong Xu.

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A patent application related to the findings of this report was filed to the patent office in Beijing, and key authors of this report were listed as inventors.

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Communicated by Neal Stewart.

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Tian, S., Jiang, L., Cui, X. et al. Engineering herbicide-resistant watermelon variety through CRISPR/Cas9-mediated base-editing. Plant Cell Rep 37, 1353–1356 (2018).

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