Evaluation of the plant growth-promoting activity of Pseudomonas nitroreducens in Arabidopsis thaliana and Lactuca sativa
Pseudomonas nitroreducens: strain IHB B 13561 (PnIHB) enhances the growth of Arabidopsis thaliana and Lactuca sativa via the stimulation of cell development and nitrate absorption.
Plant growth-promoting rhizobacteria (PGPR) enhance plant development through various mechanisms; they improve the uptake of soil resources by plants to greatly promote plant growth. Here, we used Arabidopsis thaliana seedlings and Lactuca sativa to screen the growth enhancement activities of a purified PGPR, Pseudomonas nitroreducens strain IHB B 13561 (PnIHB). When cocultivated with PnIHB, both species of plants exhibited notably improved growth, particularly in regard to biomass. Quantitative reverse transcription polymerase chain reaction analysis indicated high expression levels of the nitrate transporter genes, especially NRT2.1, which plays a major role in the high-affinity nitrate transport system in roots. Moreover, enhanced activity of the cyclin-B1 promoter was observed when wild-type ‘Columbia-0’ Arabidopsis seedlings were exposed to PnIHB, whereas upregulation of cyclin-B also occurred in the inoculated lettuce seedlings. Overall, these results suggest that PnIHB improves A. thaliana and L. sativa growth via specific pathways involved in the promotion of cell development and enhancement of nitrate uptake.
KeywordsArabidopsis thaliana CycB1 Lactuca sativa PGPR PnIHB NRT2
Financial support for this work was provided through a grant from the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (to H.L., 2016; Grant #2016-116118-3).
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Conflict of interest
The authors declare that they have no conflicts of interest.
- Poitout A, Martinière A, Kucharczyk B, Queruel N, Silva-Andia J, Mashkoor S, Gamet L, Varoquax F, Paris N, Sentenac H, Touraine B, Desbrosses G (2017) Local signalling pathways regulate the Arabidopsis root developmental response to Mesorhizobium loti inoculation. J Exp Bot 68:1199–1211CrossRefPubMedGoogle Scholar
- Ramos-Solano B, Alga A, García-Villaraco A, García-Cristóbal J, Lucas G, Gutierrez-Manero F (2010) Biotic elicitation of isoflavone metabolism with plant growth promoting rhizobacteria in early stages of development in Glycine max var. Osumi. J Agric Food Chem 58:1484–1492CrossRefPubMedGoogle Scholar
- Sander ER (2012) Aseptic laboratory techniques: plating methods. J Vis Exp 63:3064Google Scholar
- Shir-Ly H, Hsin C, Anyi H, Nguyen T, Chang-Ping Y (2014) Draft genome sequence of Pseudomonas nitroreducens strain TX1, which degrades nonionic surfactants and estrogen-like alkylphenols. Genome Announc 2:1262–1263Google Scholar
- Yuan J, Ruan Y, Wang B, Zhang J, Wassem R, Huang Q, Shen Q (2013) Plant growth-promoting rhizobacteria strain Bacillus amyloliquefaciens NJN-6-enriched bio-organic fertilizer suppressed fusarium wilt and promoted the growth of banana plants. J Agric Food Chem 61:3774–3780CrossRefPubMedGoogle Scholar