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Plant Cell Reports

, Volume 37, Issue 5, pp 789–798 | Cite as

Sucrose is involved in the regulation of iron deficiency responses in rice (Oryza sativa L.)

  • Peng-Fei Chen
  • Lin Chen
  • Zheng-Rong Jiang
  • Gao-Peng Wang
  • Shao-Hua Wang
  • Yan-Feng Ding
Original Article

Abstract

Key message

Sucrose signaling pathways were rapidly induced in response to early iron deficiency in rice plants, and the change of sucrose contents in plants was essential for the activation of iron deficiency responses.

Abstract

Sucrose is the main product of photosynthesis in plants, and it functions not only as an energy metabolite but also a signal molecule. However, a few studies have examined the involvement of sucrose in mediating iron deficiency responses in rice. In this study, we found that the decrease in photosynthesis and total chlorophyll concentration (SPAD values) in leaves occurred at a very early stage under iron deficiency. In addition, the sucrose was increased in leaves but decreased in roots of rice plants under iron deficiency, and also the sucrose transporter (SUT) encoded genes’ expression levels in leaves were all inhibited, including OsSUT1, OsSUT2, OsSUT3, OsSUT4, and OsSUT5. The carbohydrate distribution was changed under iron deficiency and sucrose might be involved in the iron deficiency responses of rice plants. Furthermore, exogenous application of sucrose or dark treatment experiments were used to test the hypothesis; we found that the increased endogenous sucrose would cause the repression of iron acquisition-related genes in roots, while further stimulated iron transport-related genes in leaves. Compared to the exogenous application of sucrose, the dark treatment had the opposite effects. All the above results highlighted the important role of sucrose in regulating the responses of rice plants to iron deficiency.

Keywords

Rice (Oryza sativa L.) Sucrose Iron deficiency Response Regulation 

Notes

Acknowledgements

This work was supported by the Natural Science Foundation of Jiangsu Province (Grants No. BK20160716), Jiangsu Collaborative Innovation Center for Modern Crop Production, the Ministry of National Science and Technology of China (2013BAD07B09), and the National Key Research and Development Program of China (2017YFD0301200).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

299_2018_2267_MOESM1_ESM.docx (13 kb)
Supplementary material 1 (DOCX 12 KB)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Peng-Fei Chen
    • 1
    • 2
  • Lin Chen
    • 1
    • 2
    • 3
  • Zheng-Rong Jiang
    • 1
    • 2
  • Gao-Peng Wang
    • 1
    • 2
  • Shao-Hua Wang
    • 1
    • 2
    • 3
  • Yan-Feng Ding
    • 1
    • 2
    • 3
  1. 1.College of AgronomyNanjing Agricultural UniversityNanjingChina
  2. 2.Key Laboratory of Crop Physiology and Ecology in Southern ChinaMinistry of Agricultural UniversityNanjingChina
  3. 3.Jiangsu Collaborative Innovation Center for Modern Crop ProductionNanjingChina

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