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Plant Cell Reports

, Volume 37, Issue 5, pp 741–757 | Cite as

Involvement of CAT in the detoxification of HT-induced ROS burst in rice anther and its relation to pollen fertility

  • Qian Zhao
  • Lujian Zhou
  • Jianchao Liu
  • Zhenzhen Cao
  • Xiaoxia Du
  • Fudeng Huang
  • Gang Pan
  • Fangmin Cheng
Original Article

Abstract

Key message

HT-induced ROS burst in developing anther is closely related to the lowered CAT activity as the result of the markedly suppressed OsCATB transcript, thereby causing severe fertility injury for rice plants exposed to HT at meiosis stage.

Abstract

The reproductive stage of rice plants is highly sensitive to heat stress. In this paper, different rice cultivars were used to investigate the relationship of HT-induced floret sterility with reactive oxygen species (ROS) detoxification in rice anthers under well-controlled climatic conditions. Results showed that high temperature (HT) exposure significantly enhanced the ROS level and malondialdehyde (MDA) content in developing anther, and the increase in ROS amount in rice anther under HT exposure was closely associated with HT-induced decline in the activities of several antioxidant enzymes. For various antioxidant enzymes, SOD and CAT were more susceptible to the ROS burst in rice anther induced by HT exposure than APX and POD, in which SOD and CAT activity in developing anther decreased significantly by HT exposure, whereas APX activity was relatively stable among different temperature regimes. HT-induced decrease in CAT activity was attributable to the suppressed transcript of OsCATB. This occurrence was strongly responsible for HT-induced increase in ROS level and oxidative-damage in rice anther, thereby it finally caused significant reduction in pollen viability and floret fertility for the rice plants exposed to HT during meiosis. Exogenous application of 1000 µM salicylic acid (SA) may alleviate HT-induced reduction in pollen viability and floret fertility, concomitantly with the increased CAT activity and reduced ROS level in rice anther.

Keywords

High temperature Reactive oxygen species (ROS) Antioxidant enzymes CAT isoforms Floret fertility Rice (Oryza sativa L.) 

Abbreviations

APX

Ascorbate peroxidase

CAT

Catalase

CMS

Cytoplasmic male sterility

GR

Glutathione reductase

HT

High temperature

MDA

Malondialdehyde

NT

Normal temperature

PCD

Programmed cell death

POD

Peroxidase

ROS

Reactive oxygen species

SA

Salicylic acid

SOD

Superoxide dismutase

Notes

Acknowledgements

The authors are indebted to National Key Research and Development Plan of China (No. 2016YFD0300502), National Natural Science Foundation of China (No. 31571602) and Zhejiang Provincial Natural Science Foundation of China (LZ15C130001) for its financial support to this research project.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

299_2018_2264_MOESM1_ESM.docx (20 kb)
Supplementary material 1 (DOCX 19 KB)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Qian Zhao
    • 1
  • Lujian Zhou
    • 1
  • Jianchao Liu
    • 1
  • Zhenzhen Cao
    • 1
  • Xiaoxia Du
    • 1
  • Fudeng Huang
    • 2
  • Gang Pan
    • 1
  • Fangmin Cheng
    • 1
    • 3
  1. 1.Institute of Crop Science and Zhejiang Key Laboratory of Crop GermplasmZhejiang UniversityHangzhouChina
  2. 2.Institute of Crop and Nuclear Technology UtilizationZhejiang Academy of Agricultural SciencesHangzhouChina
  3. 3.Jiangsu Collaborative Innovation Center for Modern Crop ProductionNanjingChina

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