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Plant Cell Reports

, Volume 37, Issue 4, pp 665–676 | Cite as

Cell shape can be uncoupled from formononetin induction in a novel cell line from Callerya speciosa

  • Fei Qiao
  • Xue-fei Jiang
  • Han-qing Cong
  • Hua-peng Sun
  • Li Li
  • Peter Nick
Original Article

Abstract

Key message

It is the first time that formononetin produced by cell culture and its accumulation was shown to be triggered by specific stress signalling linked jasmonate pathway.

Abstract

Callerya speciosa, an endangered traditional Chinese medicine plant, is intensively used in traditional folk medicine. To develop sustainable alternatives for the overexploitation of natural resources, a suspension cell line was created from C. speciosa. Ingredients of C. speciosa, for instance the isoflavone formononetin, are formed during a peculiar swelling response of the root, which is considered as a quality trait for commercial application. A cell strain with elongated cells was obtained by using synthetic cytokinin 6-benzylaminopurine (6-BA) and synthetic auxin picloram. Both, picloram and 6-BA, promote cell division, whereas picloram was shown to be crucial for the maintenance of axial cell expansion. We addressed the question, whether the loss of axiality observed in the maturating root is necessary and sufficient for the accumulation of formononetin. While we were able to mimic a loss of axiality for cell expansion, either by specific combinations of 6-BA and picloram, or by treatment with the anti-microtubular compound oryzalin, formononetin was not detectable. However, formononetin could be induced by the stress hormone methyl jasmonate (MeJA), as well as by the bacterial elicitor flagellin peptide (flg22), but not by a necrosis inducing protein. Combined the fact that none of these treatments induced the loss of axiality, we conclude that formononetin accumulates in response to basal defence and unrelated with cell swelling.

Keywords

Callerya speciosa Cell swelling Picloram Cell line Formononetin MeJA 

Notes

Acknowledgements

This work was supported by Projects for Sino-German Cooperation on Agricultural Science and Technology (2014–2015) ‘Modernization of Traditional Chinese Medicine—A cell engineering technology of rare and precious medicinal plants’ and the Fundamental Scientific Research Funds for CATAS-TCGRI (1630032015022). We thank Prof. Dr. Zhiying Li for generating calli from stem of C. speciosa.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

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Supplementary material 1 (DOCX 26 KB)
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Supplementary material 2 (DOCX 31 KB)
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Supplementary material 3 (DOCX 14 KB)
299_2018_2259_MOESM4_ESM.docx (15 kb)
Supplementary material 4 (DOCX 14 KB)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Fei Qiao
    • 1
  • Xue-fei Jiang
    • 2
  • Han-qing Cong
    • 1
  • Hua-peng Sun
    • 1
  • Li Li
    • 1
  • Peter Nick
    • 3
  1. 1.Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture/Tropical Crops Genetic Resources InstituteChinese Academy of Tropical Agricultural SciencesDanzhouPeople’s Republic of China
  2. 2.Hainan Key Laboratory of Sustainable Utilization of Tropical Bioresources/Institute of Tropical Agriculture and ForestryHainan UniversityHaikouPeople’s Republic of China
  3. 3.Molecular Cell Biology, Botanical InstituteKarlsruhe Institute of TechnologyKarlsruheGermany

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