Plant Cell Reports

, Volume 37, Issue 4, pp 641–652 | Cite as

Cloning and characterization of a specific UDP-glycosyltransferase gene induced by DON and Fusarium graminearum

  • Lanfei Zhao
  • Xin Ma
  • Peisen Su
  • Wenyang Ge
  • Hongyan Wu
  • Xiuxiu Guo
  • Anfei Li
  • Hongwei Wang
  • Lingrang Kong
Original Article


Key message

TaUGT5: can reduce the proliferation and destruction of F. graminearum and enhance the ability of FHB resistance in wheat.


Deoxynivalenol (DON) is one of the most important toxins produced by Fusarium species that enhances the spread of the pathogen in the host. As a defense, the UDP-glycosyltransferase (UGT) family has been deduced to transform DON into the less toxic form DON-3-O-glucoside (D3G), but the specific gene member in wheat that is responsible for Fusarium head blight (FHB) resistance has been little investigated and proved. In this study, a DON and Fusarium graminearum responsive gene TaUGT5, which is specific for resistant cultivars, was cloned with a 1431 bp open reading frame (ORF) encoding 476 amino acids in Sumai3. TaUGT5 is located on chromosome 2B, which has been confirmed in nulli-tetrasomic lines of Chinese Spring (CS) and is solely expressed among three homologs on the A, B and D genomes. Over-expression of this gene in Arabidopsis conferred enhanced tolerance when grown on agar plates that contain DON. Similarly, the coleoptiles of wheat over-expressing TaUGT5 showed more resistance to F. graminearum, evidencing reduced proliferation and destruction of plant tissue by the pathogen. However, the disease resistance in spikes was not as significant as that on coleoptile compared with wild-type plants. A subcellular localization analysis revealed that TaUGT5 was localized on the plasma membrane of tobacco leaf epidermal cells. It is possible that TaUGT5 could enhance tolerance to DON, protect the plant cell from the pathogen infection and result in better maintenance of the cell structure, which slows down pathogen proliferation in plant tissue.


UDP-glycosyltransferase Fusarium graminearum Triticum aestivum Deoxynivalenol TaUGT5 



All research was performed at Shandong Agricultural University. This study was supported by the National Key Research and Development Program of China (2016YFD0100602), the National Natural Science Foundation of China (31520103911, 31471488 and 31171553) and the Transgenic Special Item, China (2016ZX08009-003-001 and 2016ZX08002003-002).

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

299_2018_2257_MOESM1_ESM.pptx (1.1 mb)
Supplementary material 1 (PPTX 1090 KB)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Lanfei Zhao
    • 1
  • Xin Ma
    • 1
  • Peisen Su
    • 1
  • Wenyang Ge
    • 1
  • Hongyan Wu
    • 2
  • Xiuxiu Guo
    • 3
  • Anfei Li
    • 1
  • Hongwei Wang
    • 1
  • Lingrang Kong
    • 1
  1. 1.State Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology, College of AgronomyShandong Agricultural UniversityTai’anPeople’s Republic of China
  2. 2.Shandong AgrUnir. Fert. SciTech. Co., LtdFeichengPeople’s Republic of China
  3. 3.Shandong Academy of Agricultural SciencesJinanPeople’s Republic of China

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