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GmMYB58 and GmMYB205 are seed-specific activators for isoflavonoid biosynthesis in Glycine max

Plant Cell Reports volume 36, pages 1889–1902 (2017)Cite this article

Abstract

Key message

GmMYB58 and GmMYB205 are key positive regulators that are involved in isoflavonoid biosynthesis in seeds of Glycine max, and they activate the expression of several structural genes in the isoflavonoid pathway.

Abstract

MYB transcription factors (TFs) are major regulators involved in flavonoid/isoflavonoid biosynthesis in many plant species. However, functions of most MYB TFs remain unknown in flavonoid/isoflavonoid pathway in Glycine max. In this study, we identified 321 MYB TFs by genome-wide searching, and further isolated and functionally characterized two MYB TFs, GmMYB58 and GmMYB205. The deduced GmMYB58 and GmMYB205 proteins contain highly conserved R2R3 repeat domain at the N-terminal region that is the signature motif of R2R3-type MYB TFs. GmMYB58 and GmMYB205 were highly expressed in early seed development stages than in the other tested organs. GmMYB58 and GmMYB205 GFP fusion proteins were found to be localized in the nucleus when they were transiently expressed in Arabidopsis thaliana mesophyll protoplast. Both GmMYB58 and GmMYB205 can activate the promoter activities of GmCHS, GmIFS2, and GmHID in the transient trans-activation assays, and the activation of GmHID by both GmMYB58 and GmMYB205 was further confirmed by yeast one-hybrid assay. In addition, over-expression of GmMYB58 and GmMYB205 resulted in significant increases in expression levels of several pathway genes in soybean hairy roots, in particular, IFS2 by more than fivefolds in GmMYB205-over-expressing lines. Moreover, isoflavonoid contents were remarkably enhanced in the GmMYB58 and GmMYB205 over-expressing hairy roots than in the control. Our results suggest that GmMYB58 and GmMYB205 are seed-specific TFs, and they can enhance isoflavonoid biosynthesis mainly through the regulation of GmIFS2 and GmHID in G. max.

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Acknowledgements

This work was supported by the National Key Research and Development Program of China (2016YFD0101005), Major State Basic Research and Development Program of China (2013CB127002), and the National Natural Science Foundation of China (31670305). We thank Ms. Yan Zhu for kind assistance with LC–MS and Mr. Guangshun Zhen for assistance in hairy root generation.

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Affiliations

  1. The Key Laboratory of Plant Resources/Beijing Botanical Garden, Institute of Botany, The Chinese Academy of Sciences, Beijing, 100093, China

    Xiaoyan Han, Qinggang Yin, Jinyue Liu, Wenbo Jiang, Shaokang Di & Yongzhen Pang

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Xiaoyan Han, Qinggang Yin & Jinyue Liu

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  1. Xiaoyan Han
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  2. Qinggang Yin
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Correspondence to Yongzhen Pang.

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Communicated by Qiao Zhao.

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Han, X., Yin, Q., Liu, J. et al. GmMYB58 and GmMYB205 are seed-specific activators for isoflavonoid biosynthesis in Glycine max . Plant Cell Rep 36, 1889–1902 (2017). https://doi.org/10.1007/s00299-017-2203-3

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Keywords

  • Glycine max
  • Hairy roots
  • Isoflavonoid biosynthesis
  • MYB transcription factors