Rapid and efficient CRISPR/Cas9 gene editing in Citrus using the YAO promoter

This is a preview of subscription content, access via your institution.

Fig. 1


  1. Aguero J, Vives Mdel C, Velazquez K, Pina JA, Navarro L, Moreno P, Guerri J (2014) Effectiveness of gene silencing induced by viral vectors based on Citrus leaf blotch virus is different in Nicotiana benthamiana and citrus plants. Virology 460–461:154–164

    Article  PubMed  Google Scholar 

  2. Benfey PN, Ren L, Chua NH (1989) The CaMV 35S enhancer contains at least two domains which can confer different developmental and tissue-specific expression patterns. EMBO J 8:2195–2202

    CAS  PubMed  PubMed Central  Google Scholar 

  3. Jia H, Wang N (2014) Targeted genome editing of sweet orange using Cas9/sgRNA. PLoS One 9:e93806

    Article  PubMed  PubMed Central  Google Scholar 

  4. Jia H, Zhang Y, Orbović V, Xu J, White FF, Jones JB, Wang N (2017) Genome editing of the disease susceptibility gene CsLOB1 in citrus confers resistance to citrus canker. Plant Biotechnol J 15(7):817–823

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  5. Li Z, Jayasankar S, Gray DJ (2001) Expression of a bifunctional green fluorescent protein (GFP) fusion marker under the control of three constitutive promoters and enhanced derivatives in transgenic grape (Vitis vinifera). Plant Sci 160:877–887

    CAS  Article  PubMed  Google Scholar 

  6. Ma X, Zhu Q, Chen Y, Liu YG (2016) CRISPR/Cas9 platforms for genome editing in plants: developments and applications. Mol Plant 9:961–974

    CAS  Article  PubMed  Google Scholar 

  7. Meng Y, Hou Y, Wang H, Ji R, Liu B, Wen J, Niu L, Lin H (2017) Targeted mutagenesis by CRISPR/Cas9 system in the model legume Medicago truncatula. Plant Cell Rep 36:371–374

    CAS  Article  PubMed  Google Scholar 

  8. Peng A, Chen S, Lei T, Xu L, He Y, Wu L, Yao L, Zou X (2017) Engineering canker-resistant plants through CRISPR/Cas9-targeted editing of the susceptibility gene CsLOB1 promoter in citrus. Plant Biotechnol J. doi:10.1111/pbi.12733

    Google Scholar 

  9. Wang X, Xu Y, Zhang S, Cao L, Huang Y, Cheng J, Wu G, Tian S, Chen C, Liu Y, Yu H, Yang X, Lan H, Wang N, Wang L, Xu J, Jiang X, Xie Z, Tan M, Larkin RM, Chen LL, Ma BG, Ruan Y, Deng X, Xu Q (2017) Genomic analyses of primitive, wild and cultivated citrus provide insights into asexual reproduction. Nat Genet 49:765–772

    CAS  Article  PubMed  Google Scholar 

  10. Wu GA, Prochnik S, Jenkins J, Salse J, Hellsten U, Murat F, Perrier X, Ruiz M, Scalabrin S, Terol J, Takita MA, Labadie K, Poulain J, Couloux A, Jabbari K, Cattonaro F, Del Fabbro C, Pinosio S, Zuccolo A, Chapman J, Grimwood J, Tadeo FR, Estornell LH, Munoz-Sanz JV, Ibanez V, Herrero-Ortega A, Aleza P, Perez-Perez J, Ramon D, Brunel D, Luro F, Chen C, Farmerie WG, Desany B, Kodira C, Mohiuddin M, Harkins T, Fredrikson K, Burns P, Lomsadze A, Borodovsky M, Reforgiato G, Freitas-Astua J, Quetier F, Navarro L, Roose M, Wincker P, Schmutz J, Morgante M, Machado MA, Talon M, Jaillon O, Ollitrault P, Gmitter F, Rokhsar D (2014) Sequencing of diverse mandarin, pummelo and orange genomes reveals complex history of admixture during citrus domestication. Nat Biotechnol 32:656–662

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  11. Xu Q, Chen LL, Ruan X, Chen D, Zhu A, Chen C, Bertrand D, Jiao WB, Hao BH, Lyon MP, Chen J, Gao S, Xing F, Lan H, Chang JW, Ge X, Lei Y, Hu Q, Miao Y, Wang L, Xiao S, Biswas MK, Zeng W, Guo F, Cao H, Yang X, Xu XW, Cheng YJ, Xu J, Liu JH, Luo OJ, Tang Z, Guo WW, Kuang H, Zhang HY, Roose ML, Nagarajan N, Deng XX, Ruan Y (2013) The draft genome of sweet orange (Citrus sinensis). Nat Genet 45:59–66

    CAS  Article  PubMed  Google Scholar 

  12. Yan L, Wei S, Wu Y, Hu R, Li H, Yang W, Xie Q (2015) High-efficiency genome editing in arabidopsis using YAO promoter-driven CRISPR/Cas9 system. Mol Plant 8:1820–1823

    CAS  Article  PubMed  Google Scholar 

Download references


This project was supported by Grant # 5200-151 from the Citrus Research Board to V.F.I. and Y.J. F.Z. was supported in part by a Yale University Brown Fellowship. We thank Dr. Qi Xie (Chinese Academy of Sciences) for sending us plasmid constructs used in this study. We also thank members of the Irish and Jacob labs for discussions and advice during the course of this work.

Author information



Corresponding authors

Correspondence to Vivian F. Irish or Yannick Jacob.

Ethics declarations

Conflict of interest

The authors declare that there are no conflicts of interest.

Additional information

Communicated by Dr. Neal Stewart.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 4990 kb)

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Zhang, F., LeBlanc, C., Irish, V.F. et al. Rapid and efficient CRISPR/Cas9 gene editing in Citrus using the YAO promoter. Plant Cell Rep 36, 1883–1887 (2017). https://doi.org/10.1007/s00299-017-2202-4

Download citation


  • Phytoene Desaturase
  • Neomycin Phosphotransferase II (NPTII)
  • Albino Phenotype
  • Transgenic Citrus
  • Mosaic Phenotype