Plant Cell Reports

, Volume 36, Issue 12, pp 1855–1869 | Cite as

Proteomics analysis identified a DRT protein involved in arsenic resistance in Populus

Original Article

Abstract

Key message

A DRT protein was identified and proved to be involved in the poplar arsenic resistance through comparative proteomics analysis between arsenic sensitive and resistant cultivars.

Abstract

Arsenic pollution in soil has been a serious problem all over the world. It is very important to dissect plants arsenic stress-response mechanisms in phytoremediation. In this study, arsenate-tolerant Populus deltoides cv. ‘zhonglin 2025’ and arsenate-sensitive Populus × euramericana cv. ‘I-45/51’ were screened from 10 poplar varieties. Systematic comparisons between these two cultivars demonstrated that ‘zhonglin 2025’ exhibited slighter morphological and structural injury, lower ROS and MDA accumulation, and higher photosynthesis and ROS scavenging ability under arsenate stress, compared with ‘I-45/51’. Through comparative proteomics analysis, we detected that most of the identified arsenate-responsive proteins were stress and defense related. Among these proteins, PdDRT102 was found to be only highly induced in ‘zhonglin 2025’ under arsenate stress. Heterologous over-expression of PdDRT102 in Arabidopsis conferred to enhanced tolerance to arsenate and sodium chloride. PdDRT102 localizes to the plasma membrane and the nucleus in Arabidopsis. Interestingly, the remarkably increased fluorescence protein signals in the nucleus were found during arsenate stress. Together, these results not only provided an overall understanding on poplar response to arsenate stress, but also revealed that DRT102 protein might involve in protecting poplar against this stress

Keywords

Poplar Proteomics PdDRT102 Arsenate stress 

Notes

Acknowledgements

This work was supported by the Sino-Africa Joint Research Project (SAJC201324).

Compliance with ethical standards

Conflict of interest

The authors declare there are no conflicts of interest.

Supplementary material

299_2017_2199_MOESM1_ESM.pdf (488 kb)
Supplementary material 1 (PDF 487 kb)
299_2017_2199_MOESM2_ESM.xlsx (95 kb)
Supplementary material 2 (XLSX 95 kb)
299_2017_2199_MOESM3_ESM.pdf (150 kb)
Supplementary material 3 (PDF 150 kb)

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Yanli Liu
    • 1
    • 2
  • Rebecca Njeri Damaris
    • 1
  • Pingfang Yang
    • 1
    • 3
    • 4
  1. 1.Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical GardenThe Chinese Academy of SciencesWuhanChina
  2. 2.Institute of Fruit and TeaHubei Academy of Agricultural ScienceWuhanChina
  3. 3.Sino-African Joint Research CenterChinese Academy of SciencesWuhanChina
  4. 4.Hubei Collaborative Innovation Center for Grain IndustryHubeiChina

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