Skip to main content
SpringerLink
Log in

Precise oligonucleotide-directed mutagenesis of the Chlamydomonas reinhardtii genome

  • Focus Article
  • Published:
Plant Cell Reports Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  • Baek K, Kim DH, Jeong J, Sim SJ, Melis A, Kim JS, Jin E, Bae S (2016) DNA-free two-gene knockout in Chlamydomonas reinhardtii via CRISPR-Cas9 ribonucleoproteins. Sci Rep 6:30620. doi:10.1038/srep30620.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Jiang W, Brueggeman AJ, Horken KM, Plucinak TM, Weeks DP (2014) Successful transient expression of Cas9 and single guide RNA genes in Chlamydomonas reinhardtii. Eukaryot. Cell 13:1465–1479

    Google Scholar 

  • Kovar JL, Zhang J, Funke RP, Weeks DP (2002) Molecular analysis of the acetolactate synthase gene of Chlamydomonas reinhardtii and development of a genetically engineered gene as a dominant selectable marker for genetic transformation. Plant J 29:109–117

    Article  CAS  PubMed  Google Scholar 

  • Mages W, Heinrich O, Treuner G, Vlcek D, Daubnerova I, Slaninova M (2007) Complementation of the Chlamydomonas reinhardtii arg7-8 (arg2) point mutation by recombination with a truncated nonfunctional ARG7 gene. Protist 158:435–446

    Article  CAS  PubMed  Google Scholar 

  • Sauer NJ, Mozoruk J, Miller RB, Warburg ZJ, Walker KA, Beetham PR, Schöpke CR, Gocal GF (2016) Oligonucleotide-directed mutagenesis for precision gene editing. Plant Biotechnol J 14:496–502

    Article  CAS  PubMed  Google Scholar 

  • Shin SE, Lim JM, Koh HG, Kim EK, Kang NK, Jeon S, Kwon S, Shin WS, Lee B, Hwangbo K, Kim J, Ye SH, Yun JY, Seo H, Oh HM, Kim KJ, Kim JS, Jeong WJ, Chang YK, Jeong BR (2016) CRISPR/Cas9-induced knockout and knock-in mutations in Chlamydomonas reinhardtii. Sci Rep 6:27810

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

This work was supported by funds from the University of Nebraska Foundation (Account # 01133480).

Author information

Author notes

  1. Wen-Zhi Jiang

    Present address: Department of Molecular Biology, Harvard Medical School, Cambridge, MA, 02114, USA

  2. Sarah Dumm

    Present address: Illumina, Inc., 5200 Illumina Way, San Diego, CA, 92122, USA

Authors and Affiliations

  1. Department of Biochemistry, University of Nebraska, Lincoln, NE, 68588-0664, USA

    Wen-Zhi Jiang & Donald P. Weeks

  2. Nucelis LLC, 6465 Nancy Ridge Drive, San Diego, CA, 92121, USA

    Mark E. Knuth

  3. Cibus US LLC, 6455 Nancy Ridge Drive, San Diego, CA, 92121, USA

    Sarah Dumm & Steven L. Sanders

Authors
  1. Wen-Zhi Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sarah Dumm
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mark E. Knuth
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Steven L. Sanders
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Donald P. Weeks
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Donald P. Weeks.

Ethics declarations

Conflict of interest

SS is an employee of Cibus US LLC. MK was an employee of Nucelis LLC during the course of this work and is now an employee of Cibus US LLC. SD was an employee of Cibus US LLC.

Additional information

Communicated by Neal Stewart.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Jiang, WZ., Dumm, S., Knuth, M.E. et al. Precise oligonucleotide-directed mutagenesis of the Chlamydomonas reinhardtii genome. Plant Cell Rep 36, 1001–1004 (2017). https://doi.org/10.1007/s00299-017-2138-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00299-017-2138-8

Keywords

Search

Navigation