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Over-expression of SlJA2 decreased heat tolerance of transgenic tobacco plants via salicylic acid pathway

Abstract

Key message

Over-expression of SlJA2 decreased the accumulation of SA, which resulted in significant physiological and gene expression changes in transgenic tobacco plants, leading to the decreased heat tolerance of transgenic tobacco.

Abstract

NAC family, the largest transcription factors in plants, responses to different environmental stimuli. Here, we isolated a typical NAC transcription factor (SlJA2) from tomato and got transgenic tobacco with SlJA2 over-expression. Expression of SlJA2 was induced by heat stress (42 °C), chilling stress (4 °C), drought stress, osmotic stress, abscisic acid, and salicylic acid. Over-expression of SlJA2 decreased the accumulation of salicylic acid by regulating expression of salicylic acid degradation gene under heat stress. Compared to WT plants, stomatal apertures and water loss increased in transgenic plants, and the damage of photosynthetic apparatus and chlorophyll breakdown were more serious in transgenic plants under heat stress. Meanwhile, more H2O2 and O2 ·− were accumulated transgenic plants and proline synthesis was restricted, which resulted in more serious oxidative damage compared to WT. qRT-PCR analysis showed that over-expression of SlJA2 could down-regulate genes involved in reactive oxygen species scavenging, proline biosynthesis, and response to heat stress. All the above results indicated that SlJA2 may be a negative regulator responded to plant’s heat tolerance. Thus, this study provides new insight into roles of NAC family member in plant response to abiotic stress.

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Abbreviations

ABA:

Abscisic acid

AbA:

Aureobasidin A

ABRE:

ABA-responsive elements

APX:

Ascorbate peroxidase

CaMV 35S:

Cauliflower mosaic virus 35S

CAT:

Catalase

DAB:

3,3′-Diaminobenzidine

GFP:

Green fluorescent protein

GUS:

Beta-glucuronidase

H2O2 :

Hydrogen peroxide

HPLC–MS/MS:

High-performance liquid chromatography tandem mass spectrometry

LEA:

Late embryogenesis abundant proteins

MDA:

Malondialdehyde

MV:

Methyl viologen

NADPH oxidase:

Nicotinamide adenine dinucleotide phosphate oxidase

NBT:

Nitroblue tetrazolium

O2 ·− :

Superoxide anion radical

OE:

Over-expression

PEG:

Polyethylene glycol

Pn:

Net photosynthetic rate

PSII:

Photosystem II

REL:

Relative electrolyte leakage

ROS:

Reactive oxygen species

qRT-PCR:

Real-time quantitative PCR

RWC:

Relative water content

SA:

Salicylic acid

SlJA2:

Solanum lycopersicum jasmonic acid 2

TFs:

Transcription factors

TR:

Transcription regulatory

WT:

Wild-type

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Acknowledgements

This work was supported by the State Key Basic Research and Development Plan of China (2015CB150105), the Natural Science Foundation of China (31171474, 31371553), and the Doctor Foundation of Shandong (2014BSB01031).

Author information

Correspondence to Na-Na Ma or Qing-Wei Meng.

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Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Z.-M. Liu and M.-M. Yue contributed equally to this work.

Communicated by Chun-Hai Dong.

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Liu, Z., Yue, M., Yang, D. et al. Over-expression of SlJA2 decreased heat tolerance of transgenic tobacco plants via salicylic acid pathway. Plant Cell Rep 36, 529–542 (2017). https://doi.org/10.1007/s00299-017-2100-9

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Keywords

  • Heat stress
  • SA
  • SlJA2
  • Stomatal
  • Transgenic tobacco