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Plant Cell Reports

, Volume 36, Issue 5, pp 759–772 | Cite as

Salinity induced differential methylation patterns in contrasting cultivars of foxtail millet (Setaria italica L.)

  • Garima Pandey
  • Chandra Bhan Yadav
  • Pranav Pankaj Sahu
  • Mehanathan Muthamilarasan
  • Manoj PrasadEmail author
Research Article

Abstract

Key message

Genome-wide methylation analysis of foxtail millet cultivars contrastingly differing in salinity tolerance revealed DNA demethylation events occurring in tolerant cultivar under salinity stress, eventually modulating the expression of stress-responsive genes.

Abstract

Reduced productivity and significant yield loss are the adverse effects of environmental conditions on physiological and biochemical pathways in crop plants. In this context, understanding the epigenetic machinery underlying the tolerance traits in a naturally stress tolerant crop is imperative. Foxtail millet (Setaria italica) is known for its better tolerance to abiotic stresses compared to other cereal crops. In the present study, methylation-sensitive amplified polymorphism (MSAP) technique was used to quantify the salt-induced methylation changes in two foxtail millet cultivars contrastingly differing in their tolerance levels to salt stress. The study highlighted that the DNA methylation level was significantly reduced in tolerant cultivar compared to sensitive cultivar. A total of 86 polymorphic MSAP fragments were identified, sequenced and functionally annotated. These fragments showed sequence similarity to several genes including ABC transporter, WRKY transcription factor, serine threonine-protein phosphatase, disease resistance, oxidoreductases, cell wall-related enzymes and retrotransposon and transposase like proteins, suggesting salt stress-induced methylation in these genes. Among these, four genes were chosen for expression profiling which showed differential expression pattern between both cultivars of foxtail millet. Altogether, the study infers that salinity stress induces genome-wide DNA demethylation, which in turn, modulates expression of corresponding genes.

Keywords

Methylation-sensitive amplified polymorphism (MSAP) Salt stress Methylation Epigenetics Abiotic stress Foxtail millet 

Notes

Acknowledgements

The present work is supported by the core grant of National Institute of Plant Genome Research (NIPGR), New Delhi, India. Dr. Chandra Bhan Yadav acknowledges the award of Young Scientist Research grant from Science and Engineering Research Board, Govt. of India, New Delhi. Ms. Garima Pandey and Mr. Mehanathan Muthamilarasan acknowledge the award of Senior Research Fellowship from University Grants Commission, New Delhi, India.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

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Supplementary material 8 (PPTX 2625 kb) Supplementary Fig. S1 Antioxidative enzymes assay showing changes in (a) catalase content and, (b) MDA (malondialdehyde) levels in two cultivars; IC04 tolerant and IC41 sensitive of foxtail millet in response to salt stress (250 mM NaCl) at 1 h, 3 h, 6 h, 12 h, 24 h, 48 h. Error bars indicate the standard deviation of three independent measurements, *P = 0.03, **P = 0.001, ***P < 0.0001. Supplementary Fig. S2 Blast2Go analysis for (a) biological functions, (b) cellular and (c) molecular function processes of MSAP fragments in foxtail millet. Supplementary Fig. S3 Representation of methylated and non-methylated sites for four genes (gene body) in cv. IC04 (tolerant cultivar of foxtail millet) and cv. IC041 (susceptible cultivar of foxtail millet) under salt stress treatment at 24 h and 48 h and control (non-stress) condition. (a) Transposase-related protein, (b) β-d-xylosidase, (c) Respiratory burst oxidase, (d) Xyloglucan endotransglycosylase hydrolase. Supplementary Fig. S4 Representation of methylated and non-methylated sites for four genes (upstream region) in cv. IC04 (tolerant cultivar of foxtail millet) and cv. IC041 (susceptible cultivar of foxtail millet) under salt stress treatment at 24 h and 48 h and control (non-stress) condition. (a) Transposase-related protein, (b) β-d-xylosidase, (c) Respiratory burst oxidase, (d) Xyloglucan endotransglycosylase hydrolase

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.National Institute of Plant Genome Research (NIPGR)New DelhiIndia

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