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Plant Cell Reports

, Volume 36, Issue 2, pp 297–311 | Cite as

Dynamic expression analysis of early response genes induced by potato virus Y in PVY-resistant Nicotiana tabacum

  • Shuai Chen
  • Fengxia Li
  • Dan Liu
  • Caihong Jiang
  • Lijie Cui
  • Lili Shen
  • Guanshan LiuEmail author
  • Aiguo YangEmail author
Original Article

Abstract

Key message

Dynamic transcriptional changes of the host early responses genes were detected in PVY-resistant tobacco varieties infected with Potato virus Y; PVY resistance is a complex process that needs series of stress responses.

Abstract

Potato virus Y (PVY) causes a severe viral disease in cultivated crops, especially in Solanum plants. To understand the molecular basis of plant responses to the PVY stress, suppression subtractive hybridization (SSH) and microarray approaches were combined to identify the potentially important or novel genes that were involved in early stages (12 h, 1, 2, 3, 5, 8 days) of tobacco response to PVY infection. Dynamic changes of the host plant early responses to PVY infection on a transcriptional level were detected. In total, 167 different expressed ESTs were identified. The majority of genes involved in the metabolic process were found to be down-regulated at 12 h and 1 day, and then up-regulated at least one later period. Genes related to signaling and transcriptions were almost up-regulated at 12 h, 1 or 2 days, while stress response genes were almost up-regulated at a later stage. Genes involved in transcription, transport, cell wall, and several stress responses were found to have changed expression during the PVY infection stage, and numbers of these genes have not been previously reported to be associated with tobacco PVY infection. The diversity expression of these genes indicated that PVY resistance is a complex process that needs a series of stress responses. To resist the PVY infection, the tobacco plant has numerous active and silent responses.

Keywords

Potato virus Y Suppression subtractive hybridization Microarray Early response genes Tobacco 

Abbreviations

PVY

Potato virus Y

SSH

Suppression subtractive hybridization

VAM

Virgin A mutant

KEGG

Encyclopedia of Genes and Genomes

qPCR

Real-time PCR

ERD15

Early response to dehydration 15

PI3K

Phosphoinositide 3-kinase

SERINC1

Serine incorporator 1

NPF

NRT1/PTR family proteins

CP

Virus coat protein

MDHAR

Monodehydroascorbate reductase

SOD

Superoxide dismutase

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase

SCF

SKP1-CULLIN1-F-box

RST1

RESURRECTION1

Notes

Acknowledgements

This work was supported by The Agricultural Science and Technology Innovation Program (ASTIP-TRIC01).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

299_2016_2080_MOESM1_ESM.doc (152 kb)
Supplementary material 1 (DOC 151 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Shuai Chen
    • 1
  • Fengxia Li
    • 1
  • Dan Liu
    • 1
  • Caihong Jiang
    • 1
  • Lijie Cui
    • 1
  • Lili Shen
    • 1
  • Guanshan Liu
    • 1
    Email author
  • Aiguo Yang
    • 1
    Email author
  1. 1.Tobacco Research InstituteChinese Academy of Agricultural SciencesQingdaoChina

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