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Growth increase of Arabidopsis by forced expression of rice 45S rRNA gene

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Forced expression of rice 45S rRNA gene conferred ca. 2-fold increase of above-ground growth in transgenic Arabidopsis . This growth increase was probably brought by cell proliferation, not by cell enlargement.

Abstract

Recent increase in carbon dioxide emissions is causing global climate change. The use of plant biomass as alternative energy source is one way to reduce these emissions. Therefore, reinforcement of plant biomass production is an urgent key issue to overcome both depletion of fossil energies and emission of carbon dioxide. Here, we created transgenic Arabidopsis with a 2-fold increase in above-ground growth by forced expression of the rice 45S rRNA gene using the maize ubiquitin promoter. Although the size of guard cells and ploidy of leaf-cells were similar between transgenic and control plants, numbers of stomata and pavement cells were much increased in the transgenic leaf. This data suggested that cell number, not cell expansion, was responsible for the growth increase, which might be brought by the forced expression of exogenous and full-length 45S rRNA gene. The expression level of rice 45S rRNA transcripts was very low, possibly triggering unknown machinery to enhance cell proliferation. Although microarray analysis showed enhanced expression of ethylene-responsive transcription factors, these factors might respond to ethylene induced by abiotic/biotic stresses or genomic incompatibility, which might be involved in the expression of species-specific internal transcribed spacer (ITS) sequences within rice 45S rRNA transcripts. Further analysis of the mechanism underlying the growth increase will contribute to understanding the regulation of the cell proliferation and the mechanism of hybrid vigor.

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Acknowledgements

We thank Dr. Valentine Otang Ntui for revising this manuscript.

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Authors and Affiliations

  1. Plant Cell Technology Laboratory, Graduate School of Horticulture, Chiba University, 648 Matsudo, Matsudo, Chiba, 271-8510, Japan

    So Makabe & Ikuo Nakamura

  2. Faculty of Agriculture, Shizuoka University, 836 Ohya, Shizuoka, Shizuoka, 422-8529, Japan

    Reiko Motohashi

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  1. So Makabe
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  2. Reiko Motohashi
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Correspondence to Ikuo Nakamura.

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Communicated by H. Ebinuma.

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Makabe, S., Motohashi, R. & Nakamura, I. Growth increase of Arabidopsis by forced expression of rice 45S rRNA gene. Plant Cell Rep 36, 243–254 (2017). https://doi.org/10.1007/s00299-016-2075-y

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