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We are grateful to Prof. Caixia Gao for kindly providing vectors with sgRNA scaffold and Cas9. This work was supported by grants from the Ministry of Science and Technology of the People’s Republic of China (Grant No. 2016YFD0101001) to Hao Lin and the National Natural Science Foundation of China (Grant No. 31570309) to Lifang Niu.
Conflict of interest
The authors declare that they have no conflict of interest.
Communicated by N. Stewart.
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Meng, Y., Hou, Y., Wang, H. et al. Targeted mutagenesis by CRISPR/Cas9 system in the model legume Medicago truncatula . Plant Cell Rep 36, 371–374 (2017). https://doi.org/10.1007/s00299-016-2069-9