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Plant Cell Reports

, Volume 36, Issue 1, pp 179–191 | Cite as

Heterologous expression of a novel Zoysia japonica salt-induced glycine-rich RNA-binding protein gene, ZjGRP, caused salt sensitivity in Arabidopsis

  • Ke Teng
  • Penghui Tan
  • Guozeng Xiao
  • Liebao Han
  • Zhihui ChangEmail author
  • Yuehui ChaoEmail author
Original Article

Abstract

Key message

A novel Zoysia japonica salt-induced glycine-rich RNA-binding protein gene was cloned in this study and its overexpression caused salt sensitivity in transgenic Arabidopsis.

Abstract

Glycine-rich RNA-binding proteins (GRPs) play crucial roles in diverse plant developmental processes. However, the mechanisms and functions of GRPs in salinity stress responses remain largely unknown. In this study, rapid amplification of cDNA end (RACE) PCR methods was adopted to isolate ZjGRP from Zosyia japonica, a salt-tolerant grass species. ZjGRP cDNA was 456 bp in length, corresponding to 151 amino acids. ZjGRP was localized in the nucleus and cytoplasm, and was found particularly abundantly in stomatal guard cells. Quantitative real-time PCR showed that ZjGRP was expressed in the roots, stems, and leaves of Zoysia japonica, with the greatest expression seen in the fast-growing leaves. Furthermore, expression of ZjGRP was strongly induced by treatment with NaCl, ABA, MeJA, and SA. Overexpression of ZjGRP in Arabidopsis reduced the rate of germination and retarded seedling growth. ZjGRP-overexpressing Arabidopsis thaliana exhibited weakened salinity tolerance, likely as a result of effects on ion transportation, osmosis, and antioxidation. This study indicates that ZjGRP plays an essential role in inducing salt sensitivity in transgenic plants.

Keywords

Zoysia japonica GRPs Salinity stress Transgenic Arabidopsis Gene expression 

Abbreviations

RBPs

RNA-binding proteins

qRT-PCR

Quantitative real-time polymerase chain reactions

ABA

Abscisic acid

MeJA

Methyl jasmonate

SA

Salicylic acid

GUS

Β-Glucuronidase

MDA

Malonaldehyde

ROS

Reactive oxygen species

SOD

Superoxide dismutase

POD

Peroxidases

APX

Ascorbate peroxidase

Notes

Acknowledgements

This research was supported by the National High Technology Research and Development Program of China (863 Program) (No. 2013AA102607), Knowledge Innovation Program of Shen Zhen (No.JCYJ20160331151245672) and National Natural Science Foundation of China (No.31601989 and No.31672477).

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

299_2016_2068_MOESM1_ESM.jpg (896 kb)
Figure S1. Analysis of the ZjGRP promoter sequence. (JPEG 895 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.Turfgrass Research Institute, College of ForestryBeijing Forestry UniversityBeijingChina
  2. 2.The College of Horticulture and GardenYangtze UniversityJingzhouChina

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