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Plant Cell Reports

, Volume 36, Issue 1, pp 151–162 | Cite as

Overexpression of PSK1, a SKP1-like gene homologue, from Paeonia suffruticosa, confers salinity tolerance in Arabidopsis

  • Qing Hao
  • Hongxu Ren
  • Jin Zhu
  • Liangsheng Wang
  • Shouchen Huang
  • Zheng’an Liu
  • Zhimin GaoEmail author
  • Qingyan ShuEmail author
Original Article

Abstract

Key message

Our study is the first to demonstrate that PSK1 , a SKP1 -like gene homologue, is involved in salinity tolerance. Our functional characterization of PSK1 provides new insights into tree peony development.

Abstract

A homologous gene of S-phase kinase-associated protein1 (SKP1) was cloned from tree peony (Paeonia suffruticosa) and denoted as PSK1. The 462-bp open reading frame of PSK1 was predicted to encode a protein comprising 153 amino acids, with a molecular mass of 17 kDa. The full-length gene was 1,634 bp long and included a large 904-bp intron. PSK1 transcription was detected in all tissues, with the highest level observed in sepals, followed by leaves. Under salinity stress, overexpression of PSK1 in Arabidopsis resulted in increased germination percentages, cotyledon greening, and fresh weights relative to wild-type plants. Furthermore, transgenic Arabidopsis lines containing 35S::PSK1 displayed increased expression of genes that would be essential for reproduction and growth under salinity stress: ASK1, LEAFY, FT, and CO involved in flower development and flowering time as well as P5CS, RAB18, DREB, and SOD1-3 contributing to salinity tolerance. Our functional characterization of PSK1 adds to global knowledge of the multiple functions of previously explored SKP1-like genes in plants and sheds light on the molecular mechanism underlying its role in salinity tolerance. Our findings also provide information on the function and molecular mechanism of PSK1 in tree peony flower development, thereby revealing a theoretical basis for regulation of flowering and conferral of salinity tolerance in tree peony.

Keywords

SKP1 Paeonia suffruticosa PSK1 Salinity tolerance Flowering 

Abbreviations

CaMV

Cauliflower mosaic virus

DREB

Dehydration-responsive element-binding protein

MDA

Malondialdehyde

MS

Murashige and Skoog

ORF

Open reading frame

P5CS

Δ1-Pyrroline-5-carboxylate synthase

qPCR

Quantitative real-time PCR

RAB18

Ras-related protein

RACE

Rapid amplification of cDNA ends

ROS

Reactive oxygen species

RT-PCR

Reverse transcription-PCR

SOD

Superoxide dismutase

WT

Wild type

CO

CONSTANS

FT

Flowering locus T

Notes

Acknowledgements

This study was financially supported by the National Natural Science Foundation of China (Grant Nos. 31272201 and 31471909).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

299_2016_2066_MOESM1_ESM.xlsx (12 kb)
Supplementary material 1 (XLSX 11 kb)
299_2016_2066_MOESM2_ESM.xlsx (12 kb)
Supplementary material 2 (XLSX 12 kb)
299_2016_2066_MOESM3_ESM.jpg (3 mb)
ESM_S3. Comparison of stress response of PSK1-overexpressing Arabidopsis transgenic plants (lines 7 - 9) and the WT. (A) Germination percentages of transgenic lines and WT plants calculated 1 days after keeping at 22 ºC in medium containing 0-125 mM NaCl. (B) Cotyledon greening rates of transgenic lines and WT plants growing in medium containing 0–125 mM NaCl for 1 week. (C) Root elongation measurement of 7-day-old plants maintained in medium for 1 week. Each column represents the average of three replicates, and the bar indicates SDs. The different characters above each column represent various significant differences at p < 0.05 (JPEG 3118 kb)
299_2016_2066_MOESM4_ESM.jpg (2.4 mb)
ESM_S4. Expression analysis of SOS1-3 in Arabidopsis plants (lines 7-9 and the WT) treated with 150 mM NaCl for 0–12 h. (A) SOS1 (B) SOS2 (C) SOS3. The different characters above each column represent various significant differences at p < 0.05 (JPEG 2450 kb)
299_2016_2066_MOESM5_ESM.xlsx (12 kb)
Supplementary material 5 (XLSX 12 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Qing Hao
    • 3
  • Hongxu Ren
    • 1
  • Jin Zhu
    • 1
    • 2
  • Liangsheng Wang
    • 1
  • Shouchen Huang
    • 3
  • Zheng’an Liu
    • 1
  • Zhimin Gao
    • 4
    Email author
  • Qingyan Shu
    • 1
    Email author
  1. 1.Key Laboratory of Plant Resources/Beijing Botanical Garden, Institute of BotanyChinese Academy of SciencesBeijingChina
  2. 2.University of Chinese Academy of SciencesBeijingChina
  3. 3.Landscape and Forestry CollegeQingdao Agricultural UniversityQingdaoChina
  4. 4.International Center for Bamboo and RattanKey Laboratory on the Science and Technology of Bamboo and RattanBeijingChina

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