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A novel Ap2/ERF transcription factor from Stipa purpurea leads to enhanced drought tolerance in Arabidopsis thaliana

Plant Cell Reports volume 35, pages 2227–2239 (2016)Cite this article

Abstract

Key message

SpERF1 acts as a positive regulator, contributing to drought stress tolerance in A. thaliana through activating DRE/CRT elements in the promoters of abiotic stress-responsive genes.

Abstract

Stipa purpurea is an endemic perennial grass species in alpine arid and semi-arid meadows on the Qinghai-Xizang Plateau, which is highly tolerant against drought and cold. ERF transcription factors are known to regulate gene expression under abiotic and biotic treatments. Herein, we isolated a full-length ERF gene CDS from S. purpurea named SpERF1, which was induced by drought, cold, and jasmonic acid stresses. Subcellular localization revealed that SpERF1 is a nuclear protein, consistent with its roles as a transcription factor. Overexpression of SpERF1 enhanced drought tolerance of transgenic Arabidopsis thaliana via the activation of DRE/CRT elements in the promoters of abiotic stress-responsive genes. Furthermore, increased accumulation of proline indicated that SpERF1 might be involved in proline synthesis in the transgenic lines, allowing them to have a better buffering capacity and membrane protection under drought stress. This study indicated that SpERF1 might be an attractive target in the genetic engineering for improving stress tolerance in other crops. Moreover, SpERF1 protein function analysis increased our understanding of S. purpurea’s ability to adapt to the adverse conditions of the Qinghai-Xizang Plateau.

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Acknowledgments

This work was supported financially by the National Natural Science Foundation of China (No. 41271058), the Major State Basic Research Development Program of China (No. 2010CB951704), and the Major Projects of National Natural Science Foundation of China (No. 31590823).

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    Authors and Affiliations

    1. Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Science, Kunming, 650204, China

      Yunqiang Yang, Chao Dong, Xiong Li, Jiancan Du, Min Qian, Xudong Sun & Yongping Yang

    2. Plant Germplasm and Genomics Center, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China

      Yunqiang Yang, Chao Dong, Xiong Li, Jiancan Du, Min Qian, Xudong Sun & Yongping Yang

    3. Institute of Tibetan Plateau Research at Kunming, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China

      Yunqiang Yang, Chao Dong, Xiong Li, Jiancan Du, Min Qian, Xudong Sun & Yongping Yang

    4. University of Chinese Academy of Sciences, Beijing, 100049, China

      Chao Dong & Jiancan Du

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    1. Yunqiang Yang
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    2. Chao Dong
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    Correspondence to Xudong Sun or Yongping Yang.

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    Communicated by I. Hwang.

    Y. Yang, C. Dong contributed equally to this work.

    A novel ERF transcription factor from Stipa purpurea.

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    Yang, Y., Dong, C., Li, X. et al. A novel Ap2/ERF transcription factor from Stipa purpurea leads to enhanced drought tolerance in Arabidopsis thaliana . Plant Cell Rep 35, 2227–2239 (2016). https://doi.org/10.1007/s00299-016-2030-y

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    • DOI: https://doi.org/10.1007/s00299-016-2030-y

    Keywords

    • Stipa purpurea
    • SpERF1
    • Cloning
    • Drought
    • Gene expression