Plant Cell Reports

, Volume 35, Issue 10, pp 1991–2019 | Cite as

Androgenesis, gynogenesis, and parthenogenesis haploids in cucurbit species

  • Yan-Qi Dong
  • Wei-Xing Zhao
  • Xiao-Hui Li
  • Xi-Cun Liu
  • Ning-Ning Gao
  • Jin-Hua Huang
  • Wen-Ying Wang
  • Xiao-Li Xu
  • Zhen-Hai Tang
Review

Abstract

Haploids and doubled haploids are critical components of plant breeding. This review is focused on studies on haploids and double haploids inducted in cucurbits through in vitro pollination with irradiated pollen, unfertilized ovule/ovary culture, and anther/microspore culture during the last 30 years, as well as comprehensive analysis of the main factors of each process and comparison between chromosome doubling and ploidy identification methods, with special focus on the application of double haploids in plant breeding and genetics. This review identifies existing problems affecting the efficiency of androgenesis, gynogenesis, and parthenogenesis in cucurbit species. Donor plant genotypes and surrounding environments, developmental stages of explants, culture media, stress factors, and chromosome doubling and ploidy identification are compared at length and discussed as methodologies and protocols for androgenesis, gynogenesis, and parthenogenesis in haploid and double haploid production technologies.

Keywords

Cucurbits Haploid Double haploid Pollination with irradiated pollen Unfertilized ovule/ovary culture Anther/microspore culture 

Abbreviations

2,4-D

2,4-Dichlorophenoxyacetic acid

BAP

6-Benzylaminopurine

CBM

Cucumber basal medium

CMV

Cucumber mosaic virus

CPA

Chlorophenoxyacetic acid

DH

Double haploid

DHL

Double haploid line

ELISA

Enzyme-linked immunosorbent assay

GA3

Gibberellic acid

IAA

Indole-3-acetic acid

KT

Kinetin

NAA

α-Naphthaleneacetic acid

PGR

Plant growth regulator

Put

Putrescine

RAPD

Random amplified polymorphic DNA

RFLP

Restriction fragment length polymorphism

SNP

Single nucleotide polymorphism

SSR

Simple sequence repeats

TDZ

Thidiazuron

WMV

Watermelon mosaic virus

ZYMV

Zucchini yellow mosaic virus

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Yan-Qi Dong
    • 1
  • Wei-Xing Zhao
    • 2
  • Xiao-Hui Li
    • 2
  • Xi-Cun Liu
    • 1
  • Ning-Ning Gao
    • 2
  • Jin-Hua Huang
    • 1
  • Wen-Ying Wang
    • 1
  • Xiao-Li Xu
    • 2
  • Zhen-Hai Tang
    • 1
  1. 1.Department of Vegetable Breeding and BiotechnologyXinxiang Academy of Agricultural SciencesXinxiangChina
  2. 2.Institute of HorticultureHenan Academy of Agricultural SciencesZhengzhouChina

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