CRISPR/Cas9 for plant genome editing: accomplishments, problems and prospects
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The increasing burden of the world population on agriculture requires the development of more robust crops. Dissecting the basic biology that underlies plant development and stress responses will inform the design of better crops. One powerful tool for studying plants at the molecular level is the RNA-programmed genome editing system composed of a clustered regularly interspaced short palindromic repeats (CRISPR)-encoded guide RNA and the nuclease Cas9. Here, some of the recent advances in CRISPR/Cas9 technology that have profound implications for improving the study of plant biology are described. These tools are also paving the way towards new horizons for biotechnologies and crop development.
KeywordsGenome editing Plant biotechnology Transcriptional regulation Gene targeting Synthetic biology CRISPR Cas9
Due to limited space, we could not cite the entirety of the current literature that may also be important. We thank A. Malzahn for critical reading of the manuscript and thoughtful advice on its composition and content. This work is supported by startup funds from East Carolina University and a Collaborative Funding Grant (2016-CFG-8003) from North Carolina Biotechnology Center and Syngenta to YQ.
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Conflict of interest
The authors declare that they have no conflict interests.
- Bortesi L, Fischer R (2014) The CRISPR/Cas9 system for plant genome editing and beyond. Biotechnol Adv 33:1–28Google Scholar
- Ji X, Zhang H, Zhang Y, Wang Y, Gao C (2015) Establishing a CRISPR-Cas-like immune system conferring DNA virus resistance in plants. Nat Plants 1:1–5Google Scholar
- Sauer NJ, Narváez-Vásquez J, Mozoruk J, Miller RB, Warburg ZJ, Woodward MJ, Mihiret YA, Lincoln TA, Segami RE, Sanders SL et al (2016) Oligonucleotide-mediated genome editing provides precision and function to engineered nucleases and antibiotics in plants. Plant Physiol 170:1917–1928CrossRefPubMedPubMedCentralGoogle Scholar