Plant Cell Reports

, Volume 35, Issue 6, pp 1247–1257

Spermine modulates the expression of two probable polyamine transporter genes and determines growth responses to cadaverine in Arabidopsis

  • G. H. M. Sagor
  • Thomas Berberich
  • Seiji Kojima
  • Masaru Niitsu
  • Tomonobu Kusano
Original Article

DOI: 10.1007/s00299-016-1957-3

Cite this article as:
Sagor, G.H.M., Berberich, T., Kojima, S. et al. Plant Cell Rep (2016) 35: 1247. doi:10.1007/s00299-016-1957-3

Abstract

Key message

Two genes,LAT1andOCT1, are likely to be involved in polyamine transport in Arabidopsis. Endogenous spermine levels modulate their expression and determine the sensitivity to cadaverine.

Abstract

Arabidopsis spermine (Spm) synthase (SPMS) gene-deficient mutant was previously shown to be rather resistant to the diamine cadaverine (Cad). Furthermore, a mutant deficient in polyamine oxidase 4 gene, accumulating about twofold more of Spm than wild type plants, showed increased sensitivity to Cad. It suggests that endogenous Spm content determines growth responses to Cad in Arabidopsis thaliana. Here, we showed that Arabidopsis seedlings pretreated with Spm absorbs more Cad and has shorter root growth, and that the transgenic Arabidopsis plants overexpressing the SPMS gene are hypersensitive to Cad, further supporting the above idea. The transgenic Arabidopsis overexpressing L-Amino acid Transporter 1 (LAT1) absorbed more Cad and showed increased Cad sensitivity, suggesting that LAT1 functions as a Cad importer. Recently, other research group reported that Organic Cation Transporter 1 (OCT1) is a causal gene which determines the Cad sensitivity of various Arabidopsis accessions. Furthermore, their results suggested that OCT1 is involved in Cad efflux. Thus we monitored the expression of OCT1 and LAT1 during the above experiments. Based on the results, we proposed a model in which the level of Spm content modulates the expression of OCT1 and LAT1, and determines Cad sensitivity of Arabidopsis.

Keywords

Arabidopsis Cadaverine response L-Amino acid Transporter 1 Organic Cation Transporter 1 Polyamine Spermine 

Supplementary material

299_2016_1957_MOESM1_ESM.docx (310 kb)
Supplementary material 1 (DOCX 310 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • G. H. M. Sagor
    • 1
  • Thomas Berberich
    • 2
  • Seiji Kojima
    • 1
    • 3
  • Masaru Niitsu
    • 4
  • Tomonobu Kusano
    • 1
  1. 1.Graduate School of Life SciencesTohoku UniversitySendaiJapan
  2. 2.Biodiversity and Climate Research Center, Laboratory CenterFrankfurt am MainGermany
  3. 3.Frontier Research Institute for Interdisciplinary SciencesTohoku UniversitySendaiJapan
  4. 4.Faculty of Pharmaceutical SciencesJosai UniversitySakadoJapan

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