Cell wall-related genes studies on peach cultivars with differential susceptibility to woolliness: looking for candidates as indicators of chilling tolerance

Abstract

Key message

The results obtained indicate that a β-xylosidase gene may act as good indicator of chilling tolerance and provide new insights into the complex issue of peach fruit woolliness.

Abstract

The storage of peaches at low temperatures for prolonged periods can induce a form of chilling injury (CI) called woolliness, characterized by a lack of juiciness and a mealy texture. As this disorder has been associated with abnormal cell wall dismantling, the levels of 12 transcripts encoding proteins involved in cell wall metabolism were analysed in cultivars with contrasting susceptibility to this disorder selected from five melting flesh peach cultivars. The resistant (‘Springlady’) and susceptible (‘Flordaking’) cultivars displayed differences in the level of expression of some of the selected genes during fruit softening and in woolly versus non-woolly fruits. From these genes, the level of expression of PpXyl, which encodes for a putative β-xylosidase, was the one that presented the highest correlation (negative) with the susceptibility to woolliness. PpXyl expression was also analysed in a cultivar (‘Rojo 2’) with intermediate susceptibility to woolliness, reinforcing the conclusion about the correlation of PpXyl expression to the presence of woolliness symptom. Moreover, the level of expression of PpXyl correlated to protein level detected by Western blot. Analyses of the promoter region of the PpXyl gene (1637 bp) isolated from the three cultivars showed no differences suggesting that cis-elements from other regions of the genome and/or trans elements could be responsible of the differential PpXyl expression patterns. Overall, the results obtained indicate that PpXyl may act as a good indicator of woolliness tolerance and that the regulation of expression of this gene in different cultivars does not depend on sequences upstream the coding sequence.

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Abbreviations

ACO1:

ACC (aminocyclopropane-1-carboxylic acid) oxidase 1

CI:

Chilling injury

CS:

Cold storage

EG:

Endoglucanase

Exp:

Expansin

Gal:

Galactosidase

PG:

Polygalacturonase

PL:

Pectate lyase

PME:

Pectin methylesterase

qRT-PCR:

Quantitative real-time reverse transcription-PCR

SL:

Shelf-life

SSC:

Soluble solid content

Xyl:

Xylosidase

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Acknowledgments

This work was supported by Agencia Nacional de Promoción de Actividades Científicas y Técnicas (Argentina, PICT 2010-0097). CSA, MVL, MFD and CAB are members of the Researcher Career of CONICET and MGi and LLM are fellows of the same Institution. JG and COB are members of INTA. The authors thank Drs. G. Martínez and P. Civello (IIB-INTECH) for kind gift of the anti-β-Xyl antibody.

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Correspondence to Claudia A. Bustamante.

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Communicated by A. Dhingra.

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Genero, M., Gismondi, M., Monti, L.L. et al. Cell wall-related genes studies on peach cultivars with differential susceptibility to woolliness: looking for candidates as indicators of chilling tolerance. Plant Cell Rep 35, 1235–1246 (2016). https://doi.org/10.1007/s00299-016-1956-4

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Keywords

  • Cell wall metabolism
  • Chilling injury
  • Peach
  • Prunus persica
  • Woolliness
  • Xylosidase