Functional characterization of duplicated B-class MADS-box genes in Japanese gentian
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The heterodimer formation between B-class MADS-box proteins of GsAP3a and GsPI2 proteins plays a core role for petal formation in Japanese gentian plants.
We previously isolated six B-class MADS-box genes (GsAP3a, GsAP3b, GsTM6, GsPI1, GsPI2, and GsPI3) from Japanese gentian (Gentiana scabra). To study the roles of these MADS-box genes in determining floral organ identities, we investigated protein–protein interactions among them and produced transgenic Arabidopsis and gentian plants overexpressing GsPI2 alone or in combination with GsAP3a or GsTM6. Yeast two-hybrid and bimolecular fluorescence complementation analyses revealed that among the GsPI proteins, GsPI2 interacted with both GsAP3a and GsTM6, and that these heterodimers were localized to the nuclei. The heterologous expression of GsPI2 partially converted sepals into petaloid organs in transgenic Arabidopsis, and this petaloid conversion phenomenon was accelerated by combined expression with GsAP3a but not with GsTM6. In contrast, there were no differences in morphology between vector-control plants and transgenic Arabidopsis plants expressing GsAP3a or GsTM6 alone. Transgenic gentian ectopically expressing GsPI2 produced an elongated tubular structure that consisted of an elongated petaloid organ in the first whorl and stunted inner floral organs. These results imply that the heterodimer formation between GsPI2 and GsAP3a plays a core role in determining petal and stamen identities in Japanese gentian, but other B-function genes might be important for the complete development of petal organs.
KeywordsB-class MADS-box gene Japanese gentian Modified ABC model Petal identity Transgenic plant
Author contribution statement
TN and MN conceived and designed research. TN, MS and MN conducted experiments. TN and MN analyzed data. TN and MN wrote the manuscript. All authors read and approved the manuscript.
We thank Dr. Norihiro Otsubo and Dr. Katsutomo Sasaki, NARO Institute of Floricultural Science, for SEM observations of floral surface structure, and Dr. Akira Kanno, Tohoku University, for critical advice and suggestions. We thank Ms. Akiko Kubota for producing transgenic plants. This study was supported in part by the Japan Society for the Promotion of Science KAKENHI (grant numbers 21780028 and 24780029), and the Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries and Food Industry.
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Conflict of interest
The authors declare that they have no conflict of interest.
- Nakatsuka T, Haruta KS, Pitaksutheepong C, Abe Y, Kakizaki Y, Yamamoto K, Shimada N, Yamamura S, Nishihara M (2008) Identification and characterization of R2R3-MYB and bHLH transcription factors regulating anthocyanin biosynthesis in gentian flowers. Plant Cell Physiol 49:1818–1829CrossRefPubMedGoogle Scholar
- Nishihara M, Mishiba K, Imamura T, Takahashi H, Nakatsuka T (2015) Molecular breeding of Japanese gentians—applications of genetic transformation, metabolome analyses, and genetic markers. In: Rybczyński JJ, Davey MR, Mikula A (eds) The Gentianaceae, vol 2., Biotechnology and ApplicationsSpringer, New York, pp 239–265Google Scholar
- Tsai WC, Lee PF, Chen HI, Hsiao YY, Wei WJ, Pan ZJ, Chuang MH, Kuoh CS, Chen WH, Chen HH (2005) PeMADS6, a GLOBOSA/PISTILLATA-like gene in Phalaenopsis equestris involved in petaloid formation, and correlated with flower longevity and ovary development. Plant Cell Physiol 46:1125–1139CrossRefPubMedGoogle Scholar
- van Tunen AJ, Eikelboom W, Angenent GC (1993) Floral organogenesis in Tulipa. In: Flow Newsl, p 33–37Google Scholar