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LcGST4 is an anthocyanin-related glutathione S-transferase gene in Litchi chinensis Sonn.

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Abstract

Key message

A novel LcGST4 was identified and characterized from Litchi chinensis . Expression and functional analysis demonstrated that it might function in anthocyanin accumulation in litchi.

Abstract

Glutathione S-transferases (GSTs) have been defined as detoxification enzymes for their ability to recognize reactive electrophilic xenobiotic molecules as well as endogenous secondary metabolites. Anthocyanins are among the few endogenous substrates of GSTs for vacuolar accumulation. The gene encoding a GST protein that is involved in anthocyanin sequestration from Litchi chinensis Sonn. has not been reported. Here, LcGST4, an anthocyanin-related GST, was identified and characterized. Phylogenetic analysis showed that LcGST4 was clustered with other known anthocyanin-related GSTs in the same clade. Expression analysis revealed that the expression pattern of LcGST4 was strongly correlated with anthocyanin accumulation in litchi. ABA- and light-responsive elements were found in the LcGST4 promoter, which is in agreement with the result that the expression of LcGST4 was induced by both ABA and debagging treatment. A GST activity assay in vitro verified that the LcGST4 protein shared universal activity with the GST family. Functional complementation of an Arabidopsis mutant tt19 demonstrated that LcGST4 might function in anthocyanin accumulation in litchi. Dual luciferase assay revealed that the expression of LcGST4 was activated by LcMYB1, a key R2R3-MYB transcription factor that regulates anthocyanin biosynthesis in litchi.

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Abbreviations

ABA:

Abscisic acid

ABC:

ATP binding cassette

C3G:

Cyanidin-3-O-glucoside

CDNB:

1-Chloro-2,4-dinitrobenzene

CPPU:

Forchlorfenuron

DAA:

Days after anthesis

GST:

Glutathione S-transferase

MATE:

Multidrug and toxic compound extrusion

UFGT:

UDP-glucose:flavonoid 3-O-glucosyltransferase

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Acknowledgments

The project was supported by the Special Fund for Agro-Scientific Research in the Public Interest (Project No. 20090344-5), the China Litchi and Longan Industry Technology Research System (Project No. CARS-33), the National Natural Science Fund of China (Project No. 30971985) and YangFan Innovative and Entrepreneurial Research Team Project (No. 2014YT02H013). The authors thank Satoshi Kitamura for kindly providing tt19-1 seeds, and George P. Lomonossoff for providing the pEAQ-HT plasmid.

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Correspondence to Guibing Hu.

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Communicated by K. K. Kamo.

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Hu, B., Zhao, J., Lai, B. et al. LcGST4 is an anthocyanin-related glutathione S-transferase gene in Litchi chinensis Sonn.. Plant Cell Rep 35, 831–843 (2016). https://doi.org/10.1007/s00299-015-1924-4

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