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Sunflower germin-like protein HaGLP1 promotes ROS accumulation and enhances protection against fungal pathogens in transgenic Arabidopsis thaliana

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Abstract

Key message

The novel sunflower gene HaGLP1 is the first germin-like protein characterized from the family Asteraceae. It alters the host redox status and confers protection against Sclerotinia sclerotiorum and Rhizoctonia solani.

Abstract

Germin-like proteins (GLPs) are a large, diverse and ubiquitous family of plant glycoproteins belonging to the Cupin super family. These proteins have been widely studied because of their diverse roles in important plant processes, including defence. The novel sunflower gene HaGLP1 encodes the first germin-like protein characterized from the family Asteraceae. To analyse whether constitutive in vivo expression of the HaGLP1 gene may lead to disease tolerance, we developed transgenic Arabidopsis plants that were molecularly characterized and biologically assessed after inoculation with Sclerotinia sclerotiorum or Rhizoctonia solani. HaGLP1 expression in Arabidopsis plants conferred tolerance to S. sclerotiorum at the first stages of disease and interfered with R. solani infection, thus giving rise to significant protection against the latter. Furthermore, HaGLP1 expression in Arabidopsis plants elevated endogenous ROS levels. HaGLP1-induced tolerance does not appear to be related to a constitutive induction of the plant defence or the ROS-related genes examined here. In conclusion, our data suggest that HaGLP1 is an interesting candidate for the engineering of plants with increased fungal tolerance and that this gene could also be useful for the selection of naturally overexpressing sunflower genotypes for conventional breeding purposes.

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Abbreviations

AOX1a :

Alternative oxidase 1a gene

ET:

Ethylene

GLP:

Germin-like protein

HaGLP1/HaGLP1:

Helianthus annuus germin-like protein 1 gene/protein

HvGER:

Hordeum vulgare germin-like protein

JA:

Jasmonic acid

LOX2 :

Lipoxygenase 2 gene

MLM:

Mixed-linear model

NptII:

Neomycin phosphototransferase II gene

Pnos:

Nopaline synthase promoter

PDF-1.2 :

Plant defensin 1.2 gene

PDF-1.4 :

Plant defensin 1.4 gene

PR-1 :

Pathogenesis-related protein 1 gene

ROS:

Reactive oxygen species

SA:

Salicylic acid

SOD:

Superoxide dismutase

Tnos:

Nopaline synthase terminator

VvGLP:

Vitis vinifera germin-like protein

WRKY70 :

WRKY70 transcription factor gene

WRKY25 :

WRKY25 transcription factor gene

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Acknowledgments

The authors wish to thank Ing. Dora Barreto for her valuable guidance during infection assays, and Teresa Cabrera, Matias Rodriguez, and Juan Carlos Torres for technical support. We are also grateful to Dr. Janet Higgins for helping us improve the English of the manuscript, and to Dr. Carlos Manacorda and Dr. Sebastián Asurmendi for kindly providing several primers for real-time qPCR experiments. The comments and suggestions of two anonymous reviewers are gratefully acknowledged. This research was supported by the Agencia Nacional de Promoción Científica y Tecnológica (ANPCYT PAE-31700 PID 2008 0073; PICT 2011 1365; PICT 2007 727) and the Instituto Nacional de Tecnología Agropecuaria (INTA PNBIO1131023; PNBIO1131043; AEBIO4461). VVL, RAH, CVR and NBP are career members of the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Dr. HEH is a career member of the Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CIC).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Instituto de Biotecnología, CICVyA, Instituto Nacional de Tecnología Agropecuaria (INTA)-Castelar, Dr. Nicolás Repetto y De Los Reseros S/Nº (B1686IGC) Hurlingham, Buenos Aires, Provincia De Buenos Aires, Argentina

    V. C. Beracochea, N. I. Almasia, L. Peluffo, V. Nahirñak, E. H. Hopp, N. Paniego, R. A. Heinz, C. Vazquez-Rovere & V. V. Lia

  2. Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, (EHA1428), Buenos Aires, Argentina

    E. H. Hopp, R. A. Heinz & V. V. Lia

  3. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917 (C1033AAJ), Ciudad Autónoma De Buenos Aires, Buenos Aires, Argentina

    N. Paniego, R. A. Heinz, C. Vazquez-Rovere & V. V. Lia

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  1. V. C. Beracochea
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  2. N. I. Almasia
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  3. L. Peluffo
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  4. V. Nahirñak
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Corresponding author

Correspondence to V. V. Lia.

Additional information

Communicated by C.-H. Dong.

The sequence used for this study has been deposited at GenBank under accession number KM488198.

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Beracochea, V.C., Almasia, N.I., Peluffo, L. et al. Sunflower germin-like protein HaGLP1 promotes ROS accumulation and enhances protection against fungal pathogens in transgenic Arabidopsis thaliana . Plant Cell Rep 34, 1717–1733 (2015). https://doi.org/10.1007/s00299-015-1819-4

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  • DOI: https://doi.org/10.1007/s00299-015-1819-4

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