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Overexpression of 3-deoxy-7-phosphoheptulonate synthase gene from Gossypium hirsutum enhances Arabidopsis resistance to Verticillium wilt

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Abstract

Key message

Expression of DHS1 in cotton is induced upon infection by Verticillium dahliae , and overexpression of GhDHS1 endows transgenic Arabidopsis plants excellent Verticillium resistance.

Abstract

Verticillium wilt is caused by a soil-borne fungus Verticillium dahliae. Resistance in most cotton cultivars is either scarce or unavailable, making Verticillium wilt a major obstacle in cotton production. Here, we identified a 3-deoxy-7-phosphoheptulonate synthase (DHS, EC 4.1.2.15) gene from Gossypium hirsutum, named GhDHS1. Its 1620 bp open reading frame encodes a putative 59.4 kDa protein. Phylogenetic analysis indicated that GhDHS1 is clustered in a clade with potato and tomato DHSs that can be induced by wounding and elicitors, respectively. Expression analysis demonstrated that GhDHS1 is constitutively expressed in cotton roots and stems, but transcripts are rare or non-existent in the leaves. Subcellular localization showed that GhDHS1 occurs in the plastids. When plants of three cultivars were inoculated with V. dahliae, DHS1 expression was more significantly up-regulated in the roots of resistant G. barbadense cv. Pima90-53 and G. hirsutum cv. Jimian20 than in the susceptible G. hirsutum cv. Han208. This suggested that DHS1 is involved in the cotton resistance to Verticillium wilt. Furthermore, GhDHS1 overexpressing transgenic lines of Arabidopsis were developed via Agrobacterium tumefaciens-mediated transformation. Compared with the untransformed WT (wild type), these transgenic plants showed excellent Verticillium wilt resistance with a significantly lower disease index. The overexpressing transgenic lines also had significantly longer primary roots and greatly increased xylem areas under V. dahliae infection. Overall, our results indicate that GhDHS1 performs a role in the cotton resistance to V. dahliae and would be potential to breeding cottons of Verticillium wilt resistance.

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Abbreviations

DHS/DAHPS:

3-Deoxy-7-phosphoheptulonate synthase

Dpi:

Days post-inoculation

DI:

Disease index

EST:

Expressed sequence tag

GFP:

Green fluoresce protein

Hpi:

Hours post-inoculation

ORF:

Open reading frame

RT-qPCR:

Real-time quantitative polymerase chain reaction

SSH:

Suppression subtractive hybridization

WT:

Wild type

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Acknowledgments

Financial support was received from the National Natural Science Foundation of China (No. 31171597) and the 863 Project of China (No. 2013AA102601-5).

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Hebei Agricultural University, Baoding, 071001, China

    Jun Yang, Lianlian Ji, Xingfen Wang, Yan Zhang, Lizhu Wu, Yingna Yang & Zhiying Ma

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  1. Jun Yang
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  2. Lianlian Ji
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  4. Yan Zhang
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Correspondence to Zhiying Ma.

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Communicated by J. S. Shin.

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Yang, J., Ji, L., Wang, X. et al. Overexpression of 3-deoxy-7-phosphoheptulonate synthase gene from Gossypium hirsutum enhances Arabidopsis resistance to Verticillium wilt. Plant Cell Rep 34, 1429–1441 (2015). https://doi.org/10.1007/s00299-015-1798-5

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