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Arabidopsis Qc-SNARE gene AtSFT12 is involved in salt and osmotic stress responses and Na+ accumulation in vacuoles

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Abstract

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AtSFT12, an Arabidopsis Qc-SNARE protein, is localized to Golgi organelles and is involved in salt and osmotic stress responses via accumulation of Na + in vacuoles.

Abstract

To reduce the detrimental effects of environmental stresses, plants have evolved many defense mechanisms. Here, we identified an Arabidopsis Qc-SNARE gene, AtSFT12, involved in salt and osmotic stress responses using an activation-tagging method. Both activation-tagged plants and overexpressing transgenic plants (OXs) of the AtSFT12 gene were tolerant to high concentrations of NaCl, LiCl, and mannitol, whereas loss-of-function mutants were sensitive to NaCl, LiCl, and mannitol. AtSFT12 transcription increased under NaCl, ABA, cold, and mannitol stresses but not MV treatment. GFP-fusion AtSFT12 protein was juxtaposed with Golgi marker, implying that its function is associated with Golgi-mediated transport. Quantitative measurement of Na+ using induced coupled plasma atomic emission spectroscopy revealed that AtSFT12 OXs accumulated significantly more Na+ than WT plants. In addition, Na+-dependent fluorescence analysis of Sodium Green showed comparatively higher Na+ accumulation in vacuoles of AtSFT12 OX cells than in those of WT plant cells after salt treatments. Taken together, our findings suggest that AtSTF12, a Golgi Qc-SNARE protein, plays an important role in salt and osmotic stress responses and functions in the salt stress response via sequestration of Na+ in vacuoles.

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Abbreviations

ABA:

Abscisic acid

CaMV:

Cauliflower mosaic virus

DAG:

Days after germination

GAPc:

Glyceraldehyde 3-phosphate dehydrogenase

ICP-AES:

Inductively coupled plasma atomic emission spectroscopy

MV:

Methyl viologen

RT-PCR:

Reverse-transcription PCR

SNARE:

Soluble N-ethylmaleimide-sensitive factor attachment protein receptor

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Acknowledgments

This study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2012R1A1B3001815 and 2014R1A1A2009023). This study was financially supported by the 2015 Post-Doc. Development Program of Pusan National University.

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The authors have no conflict of interest.

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    Authors and Affiliations

    1. Department of Molecular Biology, Pusan National University, Busan, 609-735, Korea

      Vaishali N. Tarte, Hye-Yeon Seok, Dong-Hyuk Woo, Dinh Huan Le, Huong T. Tran, Ji-Won Baik, In Soon Kang, Sun-Young Lee & Yong-Hwan Moon

    2. Department of Biological Sciences, Pusan National University, Busan, 609-735, Korea

      Taijoon Chung

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    1. Vaishali N. Tarte
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    Correspondence to Yong-Hwan Moon.

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    Communicated by Youn-Il Park.

    V. N. Tarte and H.-Y. Seok equally contributed to this paper.

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    Tarte, V.N., Seok, HY., Woo, DH. et al. Arabidopsis Qc-SNARE gene AtSFT12 is involved in salt and osmotic stress responses and Na+ accumulation in vacuoles. Plant Cell Rep 34, 1127–1138 (2015). https://doi.org/10.1007/s00299-015-1771-3

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