Skip to main content
SpringerLink
Log in

A ripening-induced transcription factor MaBSD1 interacts with promoters of MaEXP1/2 from banana fruit

  • Original Paper
  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Key message

The ripening-induced MaBSD1 acts as a transcriptional activator, and might be involved in banana fruit ripening partly through directly activating the expression of two ripening-associated genes, MaEXP1/2.

Abstract

BSD (BTF2-like transcription factors, synapse-associated proteins and DOS2-like proteins) transcription factors are characterized by a typical BSD domain. However, little information is available concerning their possible roles in plant growth and development, especially in fruit ripening. In the present study, one BSD gene, designated as MaBSD1, was isolated from banana fruit. MaBSD1 has an open reading frame (ORF) of 921 bp which encodes a polypeptide of 306 amino acid residues with molecular weight of 34.80 kDa, and isoelectric point (pI) of 4.54. Subcellular localization and transcriptional activation assays showed that MaBSD1 was localized in both the nucleus and cytoplasm and possessed transcriptional activity. RT-qPCR and promoter activity analysis indicated that MaBSD1 was ethylene and ripening inducible, and the accumulation of MaBSD1 transcript was correlated well with the evolution of ethylene production and ripening process. Moreover, transient assay showed that MaBSD1 could activate the expression of two cell wall modification-related genes, MaEXP1/2, via directly interacting with their promoters. Together, these data suggest that ripening-induced MaBSD1 acts as a transcriptional activator and might be associated with banana fruit ripening, at least partially through directly activating the expression of MaEXP1/2, expanding the limited information concerning the BSD transcription factor in relation to fruit ripening.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

Abbreviations

BSD:

BTF2-like transcription factors, synapse-associated proteins and DOS2-like proteins

EXP:

Expansin

GFP:

Green fluorescence protein

ORF:

Open reading frame

RT-qPCR:

Real-time quantitative polymerase chain reaction

TF:

Transcription factor

References

  • Ba LJ, Shan W, Kuang JF, Feng BH, Xiao YY, Lu WJ, Chen JY (2014) The banana MaLBD (lateral organ boundaries domain) transcription factors regulate EXPANSIN expression and are involved in fruit ripening. Plant Mol Biol Rep. doi:10.1007/s11105-014-0720-6

    Google Scholar 

  • Chen L, Zhong HY, Kuang JF, Li JG, Chen JY, Lu WJ (2011) Validation of reference genes for RT-qPCR studies of gene expression in banana fruit under different experimental conditions. Planta 234:377–390

    Article  PubMed  CAS  Google Scholar 

  • Choudhury SR, Roy S, Nag A, Singh SK, Sengupta DN (2012) Characterization of an AGAMOUS-like MADS box protein, a probable constituent of flowering and fruit ripening regulatory system in banana. PLoS One 7:e44361

    Article  Google Scholar 

  • D’Hont A, Denoeud F, Aury JM, Baurens FC, Carreel F et al (2012) The banana (Musa acuminata) genome and the evolution of monocotyledonous plants. Nature 488:213–217

    Article  PubMed  Google Scholar 

  • Doerks T, Huber S, Buchner E, Bork P (2002) BSD: a novel domain in transcription factors and synapse-associated proteins. Trends Biochem Sci 27:168–169

    Article  PubMed  CAS  Google Scholar 

  • Elitzur T, Vrebalov J, Giovannoni JJ, Goldschmidt EE, Friedman H (2010) The regulation of MADS-box gene expression during ripening of banana and their regulatory interaction with ethylene. J Exp Bot 61:1523–1535

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Fujisawa M, Nakano T, Shima Y, Ito Y (2013) A large-scale identification of direct targets of the tomato MADS box transcription factor RIPENING INHIBITOR reveals the regulation of fruit ripening. Plant Cell 25:371–386

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Hellens R, Allan A, Friel E, Bolitho K, Grafton K, Templeton M, Karunairetnam S, Gleave A, Laing W (2005) Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants. Plant Methods 1:13

    Article  PubMed  PubMed Central  Google Scholar 

  • Klee HJ, Giovannoni JJ (2011) Genetics and control of tomato fruit ripening and quality attributes. Annu Rev Genet 45:41–59

    Article  PubMed  CAS  Google Scholar 

  • Li X, Chen L, Dhaubhadel S (2012) 14-3-3 proteins regulate the intracellular localization of the transcriptional activator GmMYB176 and affect isoflavonoid synthesis in soybean. Plant J 71:239–250

    Article  PubMed  CAS  Google Scholar 

  • Mbéguié-A-Mbéguié D, Hubert O, Fils-Lycaon B, Chillet M, Baurens FC (2008) EIN3-like gene expression during fruit ripening of Cavendish banana (Musa acuminata cv. Grande Naine). Physiol Plant 133:435–448

    Article  PubMed  Google Scholar 

  • Mitsuda N, Ohme-Takagi M (2009) Functional analysis of transcription factors in Arabidopsis. Plant Cell Physiol 50:1232–1248

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Park J, Kim MJ, Jung SJ, Suh MC (2009) Identification of a novel transcription factor, AtBSD1, containing a BSD domain in Arabidopsis thaliana. J Plant Biol 52:141–146

    Article  CAS  Google Scholar 

  • Rose JKC, Cosgrove DJ, Albersheim P, Darvill AG, Bennett AB (2000) Detection of expansin proteins and activity during tomato fruit ontogeny. Plant Physiol 123:1583–1592

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Sane AVA, Sane AP, Nath P (2007) Multiple forms of a-expansin genes are expressed during banana fruit ripening and development. Postharvest Biol Tec 45:184–192

    Article  Google Scholar 

  • Shan W, Kuang JF, Chen L, Xie H, Peng HH, Xiao YY, Li XP, Chen WX, He QG, Chen JY, Lu WJ (2012) Molecular characterization of banana NAC transcription factors and their interactions with ethylene signalling component EIL during fruit ripening. J Exp Bot 63:5171–5187

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Sreedharan S, Shekhawat UKS, Ganapathi TR (2012) MusaSAP1, a A20/AN1 zinc finger gene from banana functions as a positive regulator in different stress responses. Plant Mol Biol 80:503–517

    Article  PubMed  CAS  Google Scholar 

  • Trivedi PK, Nath P (2004) MaEXP1, an ethylene-induced expansin from ripening banana fruit. Plant Sci 167:1351–1358

    Article  CAS  Google Scholar 

  • Wan CY, Wilkins TA (1994) A modified hot borate method significantly enhances the yield of high-quality RNA from cotton (Gossypium hirsutum L.). Anal Biochem 223:7–12

    Article  PubMed  CAS  Google Scholar 

  • Xiao YY, Chen JY, Kuang JF, Shan W, Xie H, Jiang YM, Lu WJ (2013) Banana ethylene response factors are involved in fruit ripening through their interactions with ethylene biosynthesis genes. J Exp Bot 64:2499–2510

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Yang ZT, Wang MJ, Sun L, Lu SJ, Bi DL et al (2014) The membrane-associated transcription factor NAC089 controls ER-stress-induced programmed cell death in plants. PLoS Genet 10(3):e1004243

    Article  PubMed  PubMed Central  Google Scholar 

  • Ye R, Yao QH, Xu ZH, Xue HW (2004) Development of an efficient method for the isolation of factors involved in gene transcription during rice embryo development. Plant J 38:348–357

    Article  PubMed  CAS  Google Scholar 

  • Yin XR, Allan AC, Chen KS, Ferguson IB (2010) Kiwifruit EIL and ERF genes involved in regulating fruit ripening. Plant Physiol 153:1280–1292

    Article  PubMed  CAS  PubMed Central  Google Scholar 

Download references

Acknowledgments

We thank Professor Seiichiro Hasezawa (Department of Integrated Biosciences, the University of Tokyo), Professor Shouyi Chen (Institute of Genetics and Developmental Biology, Chinese Academy of Sciences), and Professor Junping Gao (Department of Ornamental Horticulture, China Agricultural University) for the generous gift of tobacco BY-2 suspension cells and the transient expression vectors, respectively. This work was supported in part by the China Agriculture Research System (grant No. CARS-32-09) and Guangdong Modern Agricultural Industry Technology System (grant No. LNSG2011-12).

Conflict of interest

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China

    Liang-jie Ba, Wei Shan, Yun-yi Xiao, Jian-ye Chen, Wang-jin Lu & Jian-fei Kuang

  2. Guangdong Key Laboratory for Postharvest Science, College of Horticulture, South China Agricultural University, Guangzhou, 510642, China

    Liang-jie Ba, Wei Shan, Yun-yi Xiao, Jian-ye Chen, Wang-jin Lu & Jian-fei Kuang

Authors
  1. Liang-jie Ba
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wei Shan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yun-yi Xiao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jian-ye Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Wang-jin Lu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jian-fei Kuang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jian-fei Kuang.

Additional information

Communicated by Prakash Lakshmanan.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 2510 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ba, Lj., Shan, W., Xiao, Yy. et al. A ripening-induced transcription factor MaBSD1 interacts with promoters of MaEXP1/2 from banana fruit. Plant Cell Rep 33, 1913–1920 (2014). https://doi.org/10.1007/s00299-014-1668-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00299-014-1668-6

Keywords

Search

Navigation