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Cell wall polysaccharide distribution in Miscanthus lutarioriparius stem using immuno-detection

Plant Cell Reports volume 33, pages 643–653 (2014)Cite this article

Abstract

Key message

Cell wall polysaccharides’ occurrences in two internodes of different development stages in M. lutarioriparius stem were analyzed and three major differences between them were identified by cell wall polysaccharide probes.

Abstract

Deposition and modification of cell wall polysaccharides during stem development affect biomass yield of the Miscanthus energy crop. The distribution patterns of cell wall polysaccharides in the 2nd and the 11th internodes of M. lutarioriparius stem were studied using in situ immunofluorescence assay. Crystalline cellulose and xylan were present in most of the stem tissues except phloem, where xyloglucan was the major composition of hemicellulose. The distribution of pectin polysaccharides varied in stem tissues, particularly in vascular bundle elements. Xylogalacturonan, feruloylated-1,4-β-d-galactan and (1,3)(1,4)-β-glucans, however, were insufficient for antibodies binding in both internodes. Furthermore, the distribution of cell wall polysaccharides was differentiated in the two internodes of M. lutarioriparius. The significant differences in the pattern of occurrence of long 1,5-α-l-arabinan chain, homogalacturonan and fucosylated xyloglucans epitope were detected between the two internodes. In addition, the relationships between probable functions of polysaccharides and their distribution patterns in M. lutarioriparius stem cell wall were discussed, which would be helpful to understand the growth characteristics of Miscanthus and identify potential targets for either modification or degradation.

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Acknowledgments

LM and CBM series monoclonal antibodies were general gifts from Prof. J. Paul Knox (Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, United Kingdom), and JIM and CCRC series monoclonal antibodies were gifts from Prof. Michael G. Hahn (Complex Carbohydrate Research Center, University of Georgia, USA). We would like to thank them for kindly providing monoclonal antibodies. The authors like to thank Amy Mason (Forage Improvement Division, the Samuel Roberts Noble Foundation, USA) for her critical review on the manuscript. Financial support for this work was obtained from the National Science and Technology Support Program (2013BAD22B01), the National Basic Research Program of China (2012CB114501), the National High-Tech Research and Development Program of China (2011AA100209) and the Program of 100 Distinguished Young Scientists of the Chinese Academy of Sciences (to Chunxiang Fu).

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Affiliations

  1. Key Laboratory of Biofuels, Chinese Academy of Sciences, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences (QIBEBT-CAS), Qingdao, 266101, Shandong, People’s Republic of China

    Yingping Cao, Junling Li, Li Yu, Guohua Chai, Guo He, Ruibo Hu, Guang Qi, Chunxiang Fu & Gongke Zhou

  2. Key Laboratory of Tobacco Genetic Improvement and Biotechnology, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, People’s Republic of China

    Yingzhen Kong

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  1. Yingping Cao
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  2. Junling Li
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  3. Li Yu
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  5. Guo He
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  7. Guang Qi
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  8. Yingzhen Kong
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  9. Chunxiang Fu
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  10. Gongke Zhou
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Correspondence to Chunxiang Fu or Gongke Zhou.

Additional information

Y. Cao and J. Li contributed equally to the article.

Communicated by Q. Zhao.

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Cao, Y., Li, J., Yu, L. et al. Cell wall polysaccharide distribution in Miscanthus lutarioriparius stem using immuno-detection. Plant Cell Rep 33, 643–653 (2014). https://doi.org/10.1007/s00299-014-1574-y

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  • DOI: https://doi.org/10.1007/s00299-014-1574-y

Keywords

  • Biofuel
  • Cell wall
  • Glycan-directed probes
  • In situ immunofluorescence assay
  • M. lutarioriparius