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Differential induction of oxylipin pathway in potato and tobacco cells by bacterial and oomycete elicitors

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Abstract

Key message

Potato and tobacco cells are differentially suited to study oxylipin pathway and elicitor-induced responses.

Abstract

Synthesis of oxylipins via the lipoxygenase (LOX) pathway provides plant cells with an important class of signaling molecules, related to plant stress responses and innate immunity. The aim of this study was to evaluate the induction of LOX pathway in tobacco and potato cells induced by a concentrated culture filtrate (CCF) from Phytophthora infestans and lipopolysaccharide (LPS) from Pectobacterium atrosepticum. Oxylipin activation was evaluated by the measurement of LOX activity and metabolite quantification. The basal levels of oxylipins and fatty acids showed that potato cells contained higher amounts of linoleic (LA), linolenic (LnA) and stearic acids than tobacco cells. The major oxylipin in potato cells, 9(S),10(S),11(R)-trihydroxy-12(Z),15(Z)-octadecadienoic acid (9,10,11-THOD), was not detected in tobacco cells. CCF induced a sharp increase of LA and LnA at 8 h in tobacco cells. In contrast they decreased in potato cells. In CCF-treated tobacco cells, colneleic acid increased up to 24 h, colnelenic acid and 9(S)-hydroxyoctadecatrienoic acid (9(S)-HOT) increased up to 16 h. In potato cells, only colneleic acid increased slightly until 16 h. A differential induction of LOX activity was measured in both cells treated by CCF. With LPS treatment, only 9,10,11-THOD accumulation was significantly induced at 16 h in potato cells. Fatty acids were constant in tobacco but decreased in potato cells over the studied time period. These results showed that the two elicitors were differently perceived by the two Solanaceae and that oxylipin pathway is strongly induced in tobacco with the CCF. They also revealed that elicitor-induced responses depended on both cell culture and elicitor.

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Abbreviations

CCF:

Concentrated culture filtrate

Cv:

Cultivar

HOD:

Hydroxy octadecadienoic acid

HOE:

Hydroxy octadecenoic acid

HOT:

Hydroxy octadecatrienoic acid

HPO:

Hydroperoxide

HPOD:

Hydroperoxy octadecadienoic acid

HPOT:

Hydroperoxy octadecatrienoic acid

HR:

Hypersensitive response

LAH:

Lipid acyl hydrolase

LOX:

Lipoxygenase

LPS:

Lipopolysaccharide

PAL:

Phenylalanine ammonia-lyase

PLA:

Phospholipase

Pv:

Pathovar

PUFA:

Polyunsaturated fatty acid

ROS:

Reactive oxygen species

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Acknowledgments

This work was funded through funds from the “Région Bretagne” PRIR Program, France.

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Authors and Affiliations

  1. INRA, UMR1349 IGEPP, 35653, Le Rheu, France

    Guillaume Saubeau & Didier Andrivon

  2. UMR 7139 CNRS-UPMC, Station Biologique, Place Georges Teissier, 29682, Roscoff, France

    Sophie Goulitquer & Philippe Potin

  3. AGROCAMPUS OUEST, UMR 0985 ESE, 35000, Rennes, France

    Dominique Barloy

  4. AGROCAMPUS OUEST, UMR1349 IGEPP, 35000, Rennes, France

    Florence Val

  5. Université Européenne de Bretagne, Rennes, France

    Dominique Barloy & Florence Val

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  1. Guillaume Saubeau
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  2. Sophie Goulitquer
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  3. Dominique Barloy
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  4. Philippe Potin
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  5. Didier Andrivon
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  6. Florence Val
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Correspondence to Florence Val.

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Communicated by M. Petersen.

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Saubeau, G., Goulitquer, S., Barloy, D. et al. Differential induction of oxylipin pathway in potato and tobacco cells by bacterial and oomycete elicitors. Plant Cell Rep 32, 579–589 (2013). https://doi.org/10.1007/s00299-012-1377-y

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  • DOI: https://doi.org/10.1007/s00299-012-1377-y

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