Antioxidant activity of seven leafy vegetables and four beans against five reactive oxygen species and reactive nitrogen species was clearly characterized with a protocol using myoglobin as a reporter probe.
Antioxidant activity of seven leafy vegetables and four beans against peroxyl radical, hydroxyl radical, hypochlorite ion, and peroxynitrite ion has been measured using myoglobin as a reporter probe (myoglobin method). Conventional DPPH method was also used to evaluate antioxidant activity of the samples. Difference of activity against different reactive oxygen species (ROS) and reactive nitrogen species (RNS) was characterized by plotting the data in a 5-axe cobweb chart. This plot clearly showed the characteristics of the antioxidant activity of the leafy vegetables and the beans. The samples examined in this work were categorized into four groups. (1) The samples showed high antioxidant activity against all ROS and RNS: daikon sprout, spinach, Qing-geng-cai, and onion. (2) The samples showed high antioxidant activity against peroxyl radical: red bean and soy bean. (3) The samples showed high antioxidant against hypochlorite ion: broccoli floret, cabbage, and Chinese cabbage. (4) The samples showed weak antioxidant activity against all ROS and RNS: cowpea and common beans. Our protocol is probably useful to characterize antioxidant activity of the crops of different cultivars, the crops obtained in different growing environments and growing seasons, the crops harvested at different age, and the crops stored in the different conditions, as well as the changes of activity during cooking process of the crops.
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Açar ÖÇ, Gökmen V, Pellegrini N, Fogliano V (2009) Direct evaluation of the total antioxidant capacity of raw and roasted pulses, nuts and seeds. Eur Food Res Technol 229:961–969
Ashfield-Watt PA, Welch AA, Day NE, Bingham SA (2004) Is ‘five-a-day’ an effective way of increasing fruit and vegetable intakes? Public Health Nutr 7:257–261
Blois MS (1958) Antioxidant determinations by the use of a stable free radical. Nature 181:1199–1200
Chen K, Keaney JF Jr (2012) Evolving concepts of oxidative stress and reactive oxygen species in cardiovascular disease. Curr Atheroscler Rep 14:476–483
Corcoran MP, McKay DL, Blumberg JB (2012) Flavonoid basics: chemistry, sources, mechanisms of action, and safety. J Nutr Gerontol Geriatr 31:176–189
Dhar SK, St Clair DK (2012) Manganese superoxide dismutase regulation and cancer. Free Radic Biol Med 52:2209–2222
Dröge W (2003) Oxidative stress and aging. Adv Exp Med Biol 543:191–200
Gökmen V, Serpen A, Fogliano V (2009) Direct measurement of the total antioxidant capacity of foods: a ‘QUENCHER’ approach. Trends Food Sci Technol 20:278–288
Gonzalez E, Vaillant F, Rojas G, Perez A (2010) Novel semiautomated method for assessing in vitro cellular antioxidant activity using light-scattering properties of human erythrocytes. J Agric Food Chem 58:1455–1461
Gorjanovic SZ, Novakovic MM, Potkonjak NI, LeskoSek-Cukalovic I, Suznjevic DZ (2010) Application of a novel antioxidative assay in beer analysis and brewing process monitoring. J Agric Food Chem 58:744–751
Kim JY, Hong JH, Jung HK, Jeong YS, Cho KH (2012) Grape skin and loquat leaf extracts and acai puree have potent anti-atherosclerotic and anti-diabetic activity in vitro and in vivo in hypercholesterolemic zebrafish. Int J Mol Med 30:606–614
Madamanchi NR, Vendrov A, Runge MS (2005) Oxidative stress and vascular disease. Arterioscler Thromb Vasc Biol 25:29–38
Matough FA, Budin SB, Hamid ZA, Alwahaibi N, Mohamed J (2012) The role of oxidative stress and antioxidants in diabetic complications. Sultan Qaboos Univ Med J 12:5–18
Maynard M, Gunnell D, Emmett P, Frankel S, Davey Smith G (2003) Fruit, vegetables, and antioxidants in childhood and risk of adult cancer: the Boyd Orr cohort. J Epidemiol Community Health 57:218–225
Munoz–Munoz JL, Garcia-Molina F, Varon R, Tudela J, Garcia-Canovas F, Rodriguez-Lopez JN (2010) Quantification of the antioxidant capacity of different molecules and their antioxidant efficiencies. J Agric Food Chem 58:2062–2070
Niki E (2010) Assessment of antioxidant capacity in vitro and in vivo. Free Radic Biol Med 49:503–515
Palmer DM, Kitchin JS (2010) Oxidative damage, skin aging, antioxidants and a novel antioxidant rating system. J Drugs Dermatol 9:11–15
Saba AB, Onakoya OM, Oyagbemi AA (2012) Hepatoprotective and in vivo antioxidant activities of ethanolic extract of whole fruit of Lagenaria breviflora. J Basic Clin Physiol Pharmacol 23:27–32
Serpen A, Capuano E, Fogliano V, Gökmen V (2007) A new procedure to measure the antioxidant activity of insoluble food components. J Agric Food Chem 55:7676–7681
Serpen A, Gökmen V, Pellegrini N, Fogliano V (2008) Direct measurement of the total antioxidant capacity of cereal products. J Cereal Sci 48:816–820
Sykes PA, Shiue H-C, Walker JR, Bateman RC Jr (1999) Determination of myoglobin stability by visible spectroscopy. J Chem Educ 76:1283–1284
Terashima M, Nakatani I, Harima A, Nakamura S, Shiiba M (2007) New method to evaluate water-soluble antioxidant activity based on protein structure change. J Agric Food Chem 55:165–169
Terashima M, Watanabe R, Ueki M, Matsumura S (2010) Comprehensive evaluation of antioxidant activity for various substances with 5-axe Cobweb chart. Food Chem 120:150–155
Terashima M, Kakuno Y, Kitano N, Matsuoka C, Murase M, Togo N, Watanabe R, Matsumura S (2012) Antioxidant activity of flavonoids evaluated with myoglobin method. Plant Cell Rep 31:291–298
Toivonen PMA, Hodges DM (2011) Leafy vegetables and salads. In: Terry LA (ed) Health-promoting properties of fruit and vegetables. CABI, Wallingford, pp 171–195
Toro J, Rodrigo R (2009) The oxidative stress: basic overview. In: Rodrigo R (ed) Oxidative stress and antioxidants: their role in human disease. Nova Biomedical Books, New York, pp 1–24
USDA Agricultural Research Services (2012) Oxygen radical absorbance capacity (ORAC) of selected foods. Release 2 (2010). http://www.ars.usda.gov/Services/docs.htm?docid=15866, last modified 05/16/2012. Accessed 10 Sept 2012
Vina J, Gambini J, Lopez-Grueso R, Abdelaziz KM, Borras C (2011) Females live longer than males: role of oxidative stress. Curr Pharm Des 17:3959–3965
Wu X, Beecher GR, Holden JM, Haytowitz DB, Gebhardt SE, Prior RL (2004a) Lipophilic and hydrophilic antioxidant capacities of common foods in the United States. J Agric Food Chem 52:4026–4037
Wu X, Gu L, Prior RL, McKay S (2004b) Characterization of anthocyanins and proanthocyanidins in some cultivars of Ribes, Aronia, and Sambucus and their antioxidant capacity. J Agric Food Chem 52:7846–7856
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Terashima, M., Fukukita, A., Kodama, R. et al. Evaluation of antioxidant activity of leafy vegetables and beans with myoglobin method. Plant Cell Rep 32, 349–357 (2013). https://doi.org/10.1007/s00299-012-1368-z