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Dramatic genotypic difference in, and effect of genetic crossing on, tissue culture-induced mobility of retrotransposon Tos17 in rice

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Abstract

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We show for the first time that intraspecific crossing may impact mobility of the prominent endogenous retrotransposon Tos17 under tissue culture conditions in rice.

Abstract

Tos17, an endogenous copia retrotransposon of rice, is transpositionally active in tissue culture. To study whether there exists fundamental genotypic difference in the tissue culture-induced mobility of Tos17, and if so, whether the difference is under genetic and/or epigenetic control, we conducted this investigation. We show that dramatic difference in tissue culture-induced Tos17 mobility exists among different rice pure-line cultivars sharing the same maternal parent: of the three lines studied that harbor Tos17, two showed mobilization of Tos17, which accrued in proportion to subculture duration, while the third line showed total quiescence (immobility) of the element and the fourth line did not contain the element. In reciprocal F1 hybrids between Tos17-mobile and -immobile (or absence) parental lines, immobility was dominant over mobility. In reciprocal F1 hybrids between both Tos17-mobile parental lines, an additive or synergistic effect on mobility of the element was noticed. In both types of reciprocal F1 hybrids, clear difference in the extent of Tos17 mobility was noted between crossing directions. Given that all lines share the same maternal parent, this observation indicates the existence of epigenetic parent-of-origin effect. We conclude that the tissue culture-induced mobility of Tos17 in rice is under complex genetic and epigenetic control, which can be either enhanced or repressed by intraspecific genetic crossing.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (No. 30970232) and the Programme for Introducing Talents to Universities (B07017).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Key Laboratory of Molecular Epigenetics of MOE, Northeast Normal University, Changchun, 130024, China

    Chunjing Lin, Lanjuan Hu, Jingjing Yang, Tianqi Zhou, Chunming Xu & Bao Liu

  2. Jilin Academy of Agricultural Sciences, Changchun, 130124, China

    Chunjing Lin, Xiuyun Lin, Likun Long, Shaochen Xing & Yingshan Dong

  3. College of Agronomy, Jilin Agricultural University, Changchun, 130118, China

    Bao Qi

Authors
  1. Chunjing Lin
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  2. Xiuyun Lin
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  3. Lanjuan Hu
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  4. Jingjing Yang
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  5. Tianqi Zhou
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  6. Likun Long
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  7. Chunming Xu
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  8. Shaochen Xing
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  9. Bao Qi
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  10. Yingshan Dong
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  11. Bao Liu
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Corresponding authors

Correspondence to Yingshan Dong or Bao Liu.

Additional information

Communicated by M. Jordan.

C. Lin and X. Lin contributed equally to this work.

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Lin, C., Lin, X., Hu, L. et al. Dramatic genotypic difference in, and effect of genetic crossing on, tissue culture-induced mobility of retrotransposon Tos17 in rice. Plant Cell Rep 31, 2057–2063 (2012). https://doi.org/10.1007/s00299-012-1316-y

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  • DOI: https://doi.org/10.1007/s00299-012-1316-y

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