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Over-expression of rice leucine-rich repeat protein results in activation of defense response, thereby enhancing resistance to bacterial soft rot in Chinese cabbage

Abstract

Pectobacterium carotovorum subsp. carotovorum causes soft rot disease in various plants, including Chinese cabbage. The simple extracellular leucine-rich repeat (eLRR) domain proteins have been implicated in disease resistance. Rice leucine-rich repeat protein (OsLRP), a rice simple eLRR domain protein, is induced by pathogens, phytohormones, and salt. To see whether OsLRP enhances disease resistance to bacterial soft rot, OsLRP was introduced into Chinese cabbage by Agrobacterium-mediated transformation. Two independent transgenic lines over-expressing OsLRP were generated and further analyzed. Transgenic lines over-expressing OsLRP showed enhanced disease resistance to bacterial soft rot compared to non-transgenic control. Bacterial growth was retarded in transgenic lines over-expressing OsLRP compared to non-transgenic controls. We propose that OsLRP confers enhanced resistance to bacterial soft rot. Monitoring expression of defense-associated genes in transgenic lines over-expressing OsLRP, two different glucanases and Brassica rapa polygalacturonase inhibiting protein 2, PDF1 were constitutively activated in transgenic lines compared to non-transgenic control. Taken together, heterologous expression of OsLRP results in the activation of defense response and enhanced resistance to bacterial soft rot.

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Abbreviations

OsLRP:

Oryza sativa leucine-rich repeat protein

Pcc:

Pectobacterium carotovorum subsp. carotovorum

HR:

Hypersensitive response

HIR:

Hypersensitive-induced reaction

Xoo:

Xanthomonas oryzae pv. oryzae

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Acknowledgments

This work was supported in part by two Grants (PJ007850 and PJ008574) from Rural Development Administration (RDA) to Duk-Ju Hwang.

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Correspondence to Duk-Ju Hwang.

Additional information

Communicated by J. S. Shin.

Y.H. Park and C. Choi contributed equally to this work.

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Park, Y.H., Choi, C., Park, E.M. et al. Over-expression of rice leucine-rich repeat protein results in activation of defense response, thereby enhancing resistance to bacterial soft rot in Chinese cabbage. Plant Cell Rep 31, 1845–1850 (2012). https://doi.org/10.1007/s00299-012-1298-9

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  • DOI: https://doi.org/10.1007/s00299-012-1298-9

Keywords

  • Rice leucine-rich repeat protein
  • Disease resistance
  • Bacterial soft rot
  • Chinese cabbage