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A new isoform of thioredoxin h group in potato, SbTRXh1, regulates cold-induced sweetening of potato tubers by adjusting sucrose content

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Abstract

In order to study the molecular mechanism of the cold-induced sweetening (CIS) of potato tubers, a novel isoform of thioredoxin h group, SbTRXh1, which was up-regulated early in the 4 °C storage of CIS-resistant potato (Solanum berthaultii) tubers, was cloned in present research. The genetic transformation of over-expression (OE) and RNA interference (RNAi) of SbTRXh1 into potato cv. E-Potato 3 (E3) was carried out to clarify its function in CIS regulation. The results showed that the transcripts of SbTRXh1 in either OE- or RNAi-tubers were strongly induced in 4 °C storage and quantitatively related to the reducing sugar (RS) accumulation, indicating that SbTRXh1 is involved in the CIS process of potato tubers. Regression analysis between the transcripts and protein contents of SbTRXh1 showed a very significant logarithmic relationship implying that the expression of SbTRXh1 may be mainly regulated at transcriptional level. Further monitoring the variation of the sugar contents in cold-stored tubers demonstrated a linear relationship between RS and sucrose (Suc). Thus, it can be inferred that SbTRXh1 may function in the Suc–RS pathway for CIS regulation of potato tubers.

Key message SbTRXh1 is primarily demonstrated to be involved in the regulation of cold-induced sweetening (CIS) of potato tubers, and it may function in the Suc–RS pathway for CIS regulation.

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Abbreviations

CIS:

Cold-induced sweetening

RS:

Reducing sugar

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Acknowledgments

This research was partially supported by grants from Teacher Foundation of Ministry of Education (20070504085), National Natural Science Foundation of China (30571181) and Earmarked Fund for Modern Agro-industry Technology Research System (CARS-10-P06).

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Authors and Affiliations

  1. National Centre for Vegetable Improvement (Central China); Key Laboratory of Horticultural Plant Biology (Huazhong Agricultural University), Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China

    Tianjiu He, Botao Song, Jun Liu, Xia Chen, Yongbin Ou, Yuan Lin, Huiling Zhang & Conghua Xie

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  1. Tianjiu He
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  2. Botao Song
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  3. Jun Liu
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  4. Xia Chen
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  5. Yongbin Ou
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  6. Yuan Lin
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  7. Huiling Zhang
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  8. Conghua Xie
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Correspondence to Conghua Xie.

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Communicated by E. Benvenuto.

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He, T., Song, B., Liu, J. et al. A new isoform of thioredoxin h group in potato, SbTRXh1, regulates cold-induced sweetening of potato tubers by adjusting sucrose content. Plant Cell Rep 31, 1463–1471 (2012). https://doi.org/10.1007/s00299-012-1261-9

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  • DOI: https://doi.org/10.1007/s00299-012-1261-9

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