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Isolation and characterization of two YUCCA flavin monooxygenase genes from cultivated strawberry (Fragaria × ananassa Duch.)

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Abstract

In strawberry (Fragaria × ananassa Duch.), auxin has been recognized as the main signal molecule coordinating the growth and initiation of ripening of fruits. The molecular mechanism regulating auxin biosynthesis in strawberry remains unknown. This project reports two YUCCA flavin monooxygenase genes FaYUC1-2 isolated from cultivated strawberry. FaYUC1 and FaYUC2 are most homologous to AtYUC6 and AtYUC4, respectively. Significant expression of FaYUC1-2 is found in vegetative meristems and reproductive organs, with overlapping but distinct patterns. During fruit development, both transcripts of FaYUC1 and FaYUC2 in achenes reach a peak around large green fruit (G2) stage, but the sudden rise in FaYUC2 transcript level is much steeper and begins earlier than that in FaYUC1. FaYUC2 is also obviously expressed in the receptacles from green fruits, hinting another auxin source for receptacle development, other than achenes. FaYUC1 over-expression Arabidopsis exhibits typical auxin hyper-accumulation phenotype in many aspects, such as the narrow and downward curled leaves, strong apical dominance, short and hairy root. It is also severely sterile, due to the disruption of floral meristems initiation and floral organs development. Transgenic analysis indicates that strawberry YUC gene may hold conserved role in auxin biosynthesis like their homologs in other plants. Integrated with the spatiotemporal expression features, these results led us to propose that FaYUC1-2 may involve in many developmental processes including flower and fruit development in strawberry.

Key message This paper is the first report of isolation and characterization of strawberry auxin biosynthesis genes. And their conserved functions in auxin biosynthesis were confirmed after ectopic expression.

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Abbreviations

FMO:

Flavin monooxygenase

ORF:

Open reading frame

RACE:

Rapid amplification of cDNA ends

RT-PCR:

Reverse transcription-PCR

SAM:

Shoot apical meristem

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Acknowledgments

The authors would like to thank anonymous referees for comments on the manuscript. This work was supported by funds from the Science and Technology Commission of Shanghai Municipality (Shanghai Rising-Star Program, 09QA1405300) and the Agricultural Commission of Shanghai Municipality (Key Program, 2008-No.1-4).

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Authors and Affiliations

  1. College of Horticultural Sciences, Nanjing Agricultural University, Nanjing, 210095, China

    Hong Liu, Zhen Zhang & Jing-Gui Fang

  2. Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Biotechnology, Shanghai Academy of Agricultural Sciences (SAAS), Beidi Road 2901, Minhang District, Shanghai, 201106, China

    Hong Liu, Yang-Yang Ying, Ling Zhang & Ke Duan

  3. Shanghai Key Laboratory of Protected Horticultural Technology, Forestry and Fruit Tree Research Institute, SAAS, Shanghai, 201403, China

    Qing-Hua Gao & Jing Li

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  1. Hong Liu
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  2. Yang-Yang Ying
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  3. Ling Zhang
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Correspondence to Jing-Gui Fang or Ke Duan.

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Communicated by H. Judelson.

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Liu, H., Ying, YY., Zhang, L. et al. Isolation and characterization of two YUCCA flavin monooxygenase genes from cultivated strawberry (Fragaria × ananassa Duch.). Plant Cell Rep 31, 1425–1435 (2012). https://doi.org/10.1007/s00299-012-1258-4

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