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Characterization and genetic analysis of an EIN4-like sequence (CaETR-1) located in QTLAR1 implicated in ascochyta blight resistance in chickpea

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Abstract

Two alleles of a chickpea (Cicer arietinum L.) ethylene receptor-like sequence (CaETR-1) were sequence-characterized using synteny analysis with genome sequences of Medicago truncatula L. The full length of the sequence obtained in the accession FLIP84-92C resistant to ascochyta blight (CaETR-1a) span 4,428 bp, including the polyadenylation signal in the 3′-untranslated region (UTR), whereas it has a 730 bp deletion in the 3′-UTR region in the susceptible accession PI359075 (CaETR-1b). The deduced protein belongs to subfamily II of the ethylene receptors and contains all the domains that define EIN4 homologs in Arabidopsis. The EIN4-like sequence (CaETR-1) has been mapped using a recombinant inbred line (RIL) population derived from an intraspecific cross between ILC3279 and WR315, resistant and susceptible to blight, respectively. The locus was located in LGIVa of the genetic map, flanked by markers NCPGR91 and GAA47 (at distances of 11.3 and 17.9 cM, respectively). This is the first potentially functional sequence identified under a QTL peak for ascochyta blight resistance in chickpea (QTLAR1). This EIN4-like (CaETR-1) sequence explained up to 33.8% of the total phenotypic variation. This sequence could be directly related to blight resistance, together with other QTLs that have been found to be involved in resistance to this major chickpea disease.

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Acknowledgments

Research was funded in part by Spanish projects AGL2005-07497-CO2-01/AGR, and by Research Grant Award No. US-3870-60C from BARD, the United States-Israel Binational Agricultural Research and Development Fund.

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Authors and Affiliations

  1. Departamento de Genética, Universidad de Córdoba, Campus Rabanales, Edif. C5, 14071, Córdoba, Spain

    E. Madrid, J. Gil & T. Millán

  2. Trait Genetics and Technologies, Dow AgroSciences, Indianapolis, IN, 46268, USA

    P. N. Rajesh

  3. Grain Legume Genetics and Physiology Research Unit, USDA-ARS, Washington State University, Pullman, WA, 99164, USA

    P. N. Rajesh & W. Chen

  4. Área de Mejora y Biotecnología, IFAPA Centro “Alameda del Obispo”, Apdo 3092, 14080, Córdoba, Spain

    J. Rubio

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  1. E. Madrid
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Correspondence to E. Madrid.

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Communicated by R. Schmidt.

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Madrid, E., Rajesh, P.N., Rubio, J. et al. Characterization and genetic analysis of an EIN4-like sequence (CaETR-1) located in QTLAR1 implicated in ascochyta blight resistance in chickpea. Plant Cell Rep 31, 1033–1042 (2012). https://doi.org/10.1007/s00299-011-1221-9

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