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Plant Cell Reports

, Volume 31, Issue 2, pp 427–436 | Cite as

Metabolite profiling of the moss Physcomitrella patens reveals evolutionary conservation of osmoprotective substances

  • Anika Erxleben
  • Arthur Gessler
  • Marco Vervliet-Scheebaum
  • Ralf Reski
Original Paper

Abstract

The moss Physcomitrella patens is suitable for systems biology studies, as it can be grown axenically under standardised conditions in plain mineral medium and comprises only few cell types. We report on metabolite profiling of two major P. patens tissues, filamentous protonema and leafy gametophores, from different culture conditions. A total of 96 compounds were detected, 21 of them as yet unknown in public databases. Protonema and gametophores had distinct metabolic profiles, especially with regard to saccharides, sugar derivates, amino acids, lignin precursors and nitrogen-rich storage compounds. A hydroponic culture was established for P. patens, and was used to apply drought stress under physiological conditions. This treatment led to accumulation of osmoprotectants, such as altrose, maltitol, ascorbic acid and proline. Thus, these osmoprotectants are not unique to seed plants but have evolved at an early phase of the colonization of land by plants.

Keywords

Bryophyte Drought stress Lignins Metabolomics Osmoprotectant Proline 

Abbreviation

GC–MS

Gas chromatography–mass spectrometry

Notes

Acknowledgments

This work was supported by the DFG Research Training Group GRK1305 “Signal Systems in Plant Model Organisms”, and by the German Federal Ministry of Education and Research BMBF (FRISYS 0313921). Support from the Freiburg Institute for Advanced Studies (FRIAS) is acknowledged.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Anika Erxleben
    • 1
    • 2
  • Arthur Gessler
    • 3
    • 4
  • Marco Vervliet-Scheebaum
    • 1
  • Ralf Reski
    • 1
    • 3
    • 5
    • 6
  1. 1.Plant BiotechnologyFaculty of Biology, University of FreiburgFreiburgGermany
  2. 2.Institute of Pharmaceutical Sciences, Pharmaceutical BioinformaticsUniversity of FreiburgFreiburgGermany
  3. 3.ZBSA, Center for Biological Systems Analysis, University of FreiburgFreiburgGermany
  4. 4.ZALF, Institute for Landscape Biogeochemistry, Leibniz-Center for Agricultural Landscape ResearchMünchebergGermany
  5. 5.FRISYS, Freiburg Initiative for Systems BiologyFreiburgGermany
  6. 6.FRIAS, Freiburg Institute for Advanced StudiesFreiburgGermany

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