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The rice thylakoid lumenal cyclophilin OsCYP20-2 confers enhanced environmental stress tolerance in tobacco and Arabidopsis

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Abstract

The role that the putative thylakoid lumenal cyclophilin (CYP) CYP20-2 locates in the thylakoid, and whether CYP20-2 is an essential gene, have not yet been elucidated. Here, we show that CYP20-2 is well conserved in several photosynthetic plants and that the transcript level of the rice OsCYP20-2 gene is highly regulated under abiotic stress. We found that ectopic expression of rice OsCYP20-2 in both tobacco and Arabidopsis confers enhanced tolerance to osmotic stress and extremely high light. Based on these results, we suggest that although the exact biochemical function of OsCYP20-2 in the thylakoid lumen (TL) remains unclear, it may be involved in photosynthetic acclimation to help plants cope with environmental stress; the OsCYP20-2 gene may be a candidate for enhancing multiple abiotic stress tolerance.

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Acknowledgments

We thank Dr. Yeonil Park (Chungnam Univ. Korea) for PEA technical assistance. This work was supported by the Biogreen 21 Project (PJ0066472010) of RDA NAAS, KRIBB Research Initiative Program and the Cabbage Genomics Assisted Breeding Supporting Center Research Programs by Ministry for Food, Agriculture, Forestry and Fisheries of the Korean Goverment to HS Cho.

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Authors and Affiliations

  1. Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Korea

    Se-Kyong Kim, Young Nim You & Hye Sun Cho

  2. Department of Biological Sciences, Seonam University, Namwon, 590-711, Korea

    Se-Kyong Kim & Jun Cheul Ahn

  3. Department of Microbiology, College of Medicine, Seonam University, Namwon, 590-170, Korea

    Jong Chun Park

  4. School of Biological Sciences and Technology, Chonnam National University, Gwangju, 500-757, Korea

    Younghee Joung

  5. Division of Bio-Crops Development, National Academy of Agricultural Science, RDA, Suwon, Korea

    Beom-Gi Kim

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  1. Se-Kyong Kim
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Correspondence to Jun Cheul Ahn or Hye Sun Cho.

Additional information

Communicated by J. S. Shin.

S.-K. Kim and Y. N. You have contributed equally in this work.

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Kim, SK., You, Y.N., Park, J.C. et al. The rice thylakoid lumenal cyclophilin OsCYP20-2 confers enhanced environmental stress tolerance in tobacco and Arabidopsis . Plant Cell Rep 31, 417–426 (2012). https://doi.org/10.1007/s00299-011-1176-x

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  • DOI: https://doi.org/10.1007/s00299-011-1176-x

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