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Plant Cell Reports

, 30:1959 | Cite as

Epigenetic repressor-like genes are differentially regulated during grapevine (Vitis vinifera L.) development

  • Rubén Almada
  • Nuri Cabrera
  • José A. Casaretto
  • Hugo Peña-Cortés
  • Simón Ruiz-Lara
  • Enrique González Villanueva
Original Paper

Abstract

Grapevine sexual reproduction involves a seasonal separation between inflorescence primordia (flowering induction) and flower development. We hypothesized that a repression mechanism implicating epigenetic changes could play a role in the seasonal separation of these two developmental processes in grapevine. Therefore, the expression of five grapevine genes with homology to the Arabidopsis epigenetic repressor genes FERTILIZATION INDEPENDENT ENDOSPERM (FIE), EMBRYONIC FLOWER 2 (EMF2), CURLY LEAF (CLF), MULTICOPY SUPPRESSOR OF IRA 1 (MSI1) and SWINGER (SWN) was analyzed during the development of buds and vegetative and reproductive organs. During bud development, the putative grapevine epigenetic repressor genes VvCLF, VvEMF2, VvMSI1, VvSWN and VvFIE are mainly expressed in latent buds at the flowering induction period, but also detected during bud burst and inflorescence/flower development. The overlapping expression patterns of grapevine PcG-like genes in buds suggest that chromatin remodeling mechanisms could be operating during grapevine bud development for controlling processes such as seasonal flowering, dormancy and bud burst. Furthermore, the expression of grapevine PcG-like genes was also detected in fruits and vegetative organs, suggesting that epigenetic changes could be at the basis of the regulation of various proliferation–differentiation cell transitions that occur during grapevine development.

Keywords

Bud development Dormancy Flowering Polycomb genes Vitis vinifera 

Notes

Acknowledgments

This work was funded by grants from Consorcio Biofrutales. R.A. and N.C. were supported by the Universidad de Talca doctoral fellowship.

Supplementary material

299_2011_1104_MOESM1_ESM.doc (66 kb)
Supporting Information Table S1 (DOC 66 kb)

References

  1. Almada RD, Cabrera N, Casaretto JA, Ruiz-Lara S, González Villanueva E (2009) VvCO and VvCOL1, two CONSTANS homologous genes, are regulated during flower induction and dormancy in grapevine buds. Plant Cell Rep 28:1193–1203PubMedCrossRefGoogle Scholar
  2. Andrade MA, Ponting C, Gibson T, Bork P (2000) Identification of protein repeats and statistical significance of sequence comparisons. J Mol Biol 298:521–537PubMedCrossRefGoogle Scholar
  3. Boss PK, Vivier M, Matsumoto S, Dry IB, Thomas MR (2001) A cDNA from grapevine (Vitis vinifera L.), which shows homology to AGAMOUS and SHATTERPROOF, is not only expressed in flowers but also throughout berry development. Plant Mol Biol 45:541–553PubMedCrossRefGoogle Scholar
  4. Boss PK, Sensi E, Hua C, Davies C, Thomas MR (2002) Cloning and characterisation of grapevine (Vitis vinifera L.) MADS-box genes expressed during inflorescence and berry development. Plant Sci 162:887–895CrossRefGoogle Scholar
  5. Calonje M, Cubas P, Martínez-Zapater JM, Carmona MJ (2004) Floral meristem identity genes are expressed during tendril development in grapevine. Plant Physiol 135:1491–1501PubMedCrossRefGoogle Scholar
  6. Carmona MJ, Cubas P, Martinez-Zapater JM (2002) VFL, the grapevine FLORICAULA/LEAFY ortholog, is expressed in meristematic regions independently of their fate. Plant Physiol 130:68–77PubMedCrossRefGoogle Scholar
  7. Carmona MJ, Cubas P, Calonje M, Martinez-Zapater JM (2007) Flowering transition in grapevine (Vitis vinifera L.). Can J Bot 85:701–711CrossRefGoogle Scholar
  8. Carmona MJ, Chaïb J, Martinez-Zapater JM, Thomas MR (2008) A molecular genetic perspective of reproductive development in grapevine. J Exp Bot 59:2579–2596PubMedCrossRefGoogle Scholar
  9. Chang S, Puryear J, Cairney J (1993) A simple and efficient method for isolating RNA from pine trees. Plant Mol Biol Rep 11:113–116CrossRefGoogle Scholar
  10. Chanvivattana Y, Bishopp A, Schubert D, Stock SC, Moon YH, Sung ZR, Goodrich J (2004) Interaction of Polycomb-group proteins controlling flowering in Arabidopsis. Development 131:5263–5276PubMedCrossRefGoogle Scholar
  11. Coombe BG (1995) Growth stages of the grapevine: adoption of a system for identifying grapevine growth stages. Aust J Grape Wine Res 1:100–110CrossRefGoogle Scholar
  12. Díaz-Riquelme J, Lijavetzky D, Martinez-Zapater JM, Carmona MJ (2009) Genome-wide analysis of MIKC-Type MADS Box in grapevine. Plant Physiol 149:354–369PubMedCrossRefGoogle Scholar
  13. Fennell A, Hoover E (1991) Photoperiod influences growth, bud dormancy and cold acclimation in Vitis labruscana and V. riparia. J Am Soc Hort Sci 116:270–273Google Scholar
  14. Henning L, Derkacheva M (2009) Diversity of Polycomb group complexes in plants: same rules, different players? Trends Genet 25:414–423CrossRefGoogle Scholar
  15. Horvath DP, Anderson JV, Chao WS, Foley ME (2003) Knowing when to grow: signals regulating bud dormancy. Trends Plant Sci 8:534–540PubMedCrossRefGoogle Scholar
  16. Horvath DP, Chao WS, Suttle JC, Thimmapuram J, Anderson JV (2008) Transcriptome analysis identifies novel responses and potential regulatory genes involved in seasonal dormancy transitions of leafy spurge (Euphorbia esula L.). BMC 9:536Google Scholar
  17. Jarillo JA, Piñeiro M, Cubas P, Martinez-Zapater JM (2009) Chromatin remodelling in plant development. Int J Dev Biol 53:1581–1596PubMedCrossRefGoogle Scholar
  18. Katz A, Oliva M, Mosquna A, Hakim O, Ohad N (2004) FIE and CURLY LEAF polycomb proteins interact in the regulation of homeobox gene expression during sporophyte development. Plant J 37:707–719PubMedCrossRefGoogle Scholar
  19. Kim SY, Zhu T, Sung ZR (2010) Epigenetic regulation of gene programs by EMF1 and EMF2 in Arabidopsis. Plant Physiol 152:516–528PubMedCrossRefGoogle Scholar
  20. Kit AH, Boureau L, Stammitti-Bert L, Rolin D, Teyssier E, Gallusci P (2010) Functional analysis of SlEZ1 a tomato Enhancer of zeste (E(z)) gene demonstrates a role in flower development. Plant Mol Biol 74:201–213CrossRefGoogle Scholar
  21. Köhler C, Hennig L (2010) Regulation of cell identity by plant Polycomb and trithorax group proteins. Curr Opin Genet Dev 20:1–7CrossRefGoogle Scholar
  22. Moon YH, Chen L, Pan RL, Chang HS, Zhu T, Maffeo DM, Sung ZR (2003) EMF genes maintain vegetative development by repressing the flower program in Arabidopsis. Plant Cell 15:681–693PubMedCrossRefGoogle Scholar
  23. Mullins MG, Bouquet A, Williams LE (1992) Biology of grapevine. Cambridge University Press, CambridgeGoogle Scholar
  24. Ng J, Li R, Morgan K, Simon J (1997) Evolutionary conservation and predicted protein structure of the Drosophila extra sex combs repressor protein. Mol Cell Biol 17:6663–6672PubMedGoogle Scholar
  25. Noriega X, Burgos B, Pérez F (2007) Short day-photoperiod triggers and low temperatures increase expression of peroxidase RNA transcripts and basic peroxidase isoenzyme activity in grapevine buds. Phytochemistry 68:1376–1383PubMedCrossRefGoogle Scholar
  26. Ohad N, Yadegari R, Margossian L, Hannon M, Michaeli D, Harada JJ, Goldberg RB, Fischer RL (1999) Mutations in FIE, a WD polycomb group gene, allow endosperm development without fertilization. Plant Cell 11:407–415PubMedCrossRefGoogle Scholar
  27. Reid KE, Olsson N, Schlosser J, Peng F, Lund ST (2006) An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development. BMC Plant Biol 6:27PubMedCrossRefGoogle Scholar
  28. Ruttink T, Arend M, Morreel K, Storme V, Rombauts S, Fromm J, Bhalerao RP, Boerjan W, Rohde A (2007) A molecular timetable for apical bud formation and dormancy induction in poplar. Plant Cell 19:2370–2390PubMedCrossRefGoogle Scholar
  29. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425PubMedGoogle Scholar
  30. Santamaría ME, Hasbún R, Valera MJ, Meijón M, Valledor L, Rodríguez JL, Toorop PE, Cañal MJ, Rodríguez R (2009) Acetylated H4 histone and genomic DNA methylation patterns during bud set and bud burst in Castanea sativa. J Plant Physiol 166:1360–1369PubMedCrossRefGoogle Scholar
  31. Schnabel BJ, Wample RL (1987) Dormancy and cold hardiness in Vitis vinifera L. cv. White Riesling as influenced by photoperiod and temperature. Am J Enol Vitic 38:265–272Google Scholar
  32. Sondek J, Boh A, Lambright DG, Hamm HE, Sigler PB (1996) Crystal structure of a GA protein dimer at 2.1 Å resolution. Nature 379:369–374PubMedCrossRefGoogle Scholar
  33. Sreekantan L, Thomas MR (2006) VvFT and VvMADS8, the grapevine homologues of the floral integrators FT and SOC1, have unique expression patterns in grapevine and hasten flowering in Arabidopsis. Funct Plant Biol 33:1129–1139CrossRefGoogle Scholar
  34. Sturn A, Quackenbush J, Trajanoski Z (2002) Genesis: cluster analysis of microarray data. Bioinformatics 18:207–208PubMedCrossRefGoogle Scholar
  35. Sung ZR, Chen L, Moon Y, Lertpiriyapong K (2003) Mechanism of Floral repression in Arabidopsis. Curr Opin Plant Biol 6:29–35PubMedCrossRefGoogle Scholar
  36. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599PubMedCrossRefGoogle Scholar
  37. van Nocker S, Ludwig P (2003) The WD-repeat protein superfamily in Arabidopsis: conservation and divergence in structure and function. BMC Genomics 4:50–60PubMedCrossRefGoogle Scholar
  38. Wake MF, Fennell A (2000) Morphological, physiological and dormancy responses of three Vitis genotypes to short photoperiod. Physiol Plant 109:203–210CrossRefGoogle Scholar
  39. Wall MA, Coleman DE, Lee E, Iniguez-Lluhi JJ, Posner BA, Gilman AG, Sprang SR (1995) The structure of the G protein heterotrimer G ia1 β1 γ2. Cell 83:1047–1058PubMedCrossRefGoogle Scholar
  40. Yadegari R, Kinoshita T, Lotan O, Cohen G, Katz A, Choi Y, Katz A, Nakashima K, Harada JJ, Goldberg RB, Fischer RL, Ohad N (2000) Mutations in the FIE and MEA genes that encode interacting polycomb proteins cause parent-of-origin effects on seed development by distinct mechanisms. Plant Cell 12:2367–2381PubMedCrossRefGoogle Scholar
  41. Yoshida N, Yanai Y, Chen L, Kato Y, Hiratsuka J, Miwa T, Sung ZR, Takahashi S (2001) EMBRYONIC FLOWER2, a novel polycomb group protein homolog, mediates shoot development and flowering in Arabidopsis. Plant Cell 13:2471–2481PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Rubén Almada
    • 1
    • 2
  • Nuri Cabrera
    • 1
  • José A. Casaretto
    • 1
  • Hugo Peña-Cortés
    • 3
  • Simón Ruiz-Lara
    • 1
  • Enrique González Villanueva
    • 1
  1. 1.Instituto de Biología Vegetal y BiotecnologíaUniversidad de TalcaTalcaChile
  2. 2.Centro de Estudios Avanzados en FruticulturaInstituto de Investigaciones Agropecuarias, CRI-RayentuéRengoChile
  3. 3.Centro de BiotecnologíaUniversidad Técnica Federico Santa MaríaValparaísoChile

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