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Evidence for non-disomic inheritance in a Citrus interspecific tetraploid somatic hybrid between C. reticulata and C. limon using SSR markers and cytogenetic analysis

Plant Cell Reports volume 30, pages 1415–1425 (2011)Cite this article

Abstract

Artificial tetraploid somatic hybrids have been developed for sterile triploid citrus breeding by sexual hybridization between diploid and tetraploid somatic hybrids. The genetic structure of diploid gametes produced by tetraploid genotypes depends on the mode of chromosome association at meiosis. In order to evaluate tetraploid inheritance in a tetraploid interspecific somatic hybrid between mandarin and lemon, we performed segregation studies using cytogenetic and single sequence repeat molecular markers. Cytogenetic analysis of meiosis in the somatic hybrid revealed 11% tetravalents and 76% bivalents. Inheritance of the tetraploid hybrid was analyzed by genotyping the triploid progeny derived from a cross between a diploid pummelo and the tetraploid somatic hybrid, in order to derive genotypes of the meiospores produced by the tetraploid. A likelihood-based approach was used to distinguish between disomic, tetrasomic, and intermediate inheritance models and to estimate the double reduction rate. In agreement with expectations based the cytogenetic data, marker segregation was largely compatible with tetrasomic and inheritance intermediate between disomic and tetrasomic, with some evidence for preferential pairing of homoeologous chromosomes. This has important implications for the design of breeding programs that involve tetraploid hybrids, and underscores the need to consider inheritance models that are intermediate between disomic and tetrasomic.

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Acknowledgments

We thank the “Domaines Abbès Kabbage”, Agadir (Morocco) and the “Comité Mixte Inter Universitaire Franco-Marocain” (Programme Volubils, MA/08/196) for the grant assigned to Mourad Kamiri. This study was supported by the “Collectivité Territoriale de Corse” (CTC).

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Affiliations

  1. Unité de Recherche Multiplication végétative, CIRAD, San Giuliano, 20230, France

    Mourad Kamiri & Yann Froelicher

  2. Domaines Abbes Kabbage, 325, Avenue Hassan II, Agadir, Morocco

    Mourad Kamiri & Ikbal Srairi

  3. Division of Ecology and Evolutionary Biology, University of Glasgow, Glasgow, G12 8QQ, Scotland, UK

    Marc Stift

  4. Centro de Investigação em Biodiversidade e Recursos Genéticos, Campus Agrário de Vairão, Rua Padre Armando Quintas, 4485-661, Vairão, Portugal

    Marc Stift

  5. Unité de Génétique et écophysiologie de la qualité des agrumes, INRA San Giuliano, F-20230, France

    Gilles Costantino

  6. Laboratoire d’Agroforestrie, Faculté des Sciences, Département de Biologie, Université Ibnou Zohr, BP 28S, Agadir, Morocco

    Abdelhamid El Moussadik

  7. Laboratoire de Biochimie, Environnement et Agroalimentaire, UFR Environnement et Santé, Faculté des Sciences et Techniques de Mohammedia, B.P.146, 20800, Mohammedia, Morocco

    Abdelaziz Hmyene

  8. Unité de Recherche: Amélioration génétique des espèces à multiplication végétative, CIRAD TA A-75/02 Avenue Agropolis, 34398, Montpellier cedex 5, France

    Frédéric Bakry & Patrick Ollitrault

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  1. Mourad Kamiri
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  2. Marc Stift
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  8. Patrick Ollitrault
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  9. Yann Froelicher
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Correspondence to Yann Froelicher.

Additional information

Communicated by D. Somers.

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Kamiri, M., Stift, M., Srairi, I. et al. Evidence for non-disomic inheritance in a Citrus interspecific tetraploid somatic hybrid between C. reticulata and C. limon using SSR markers and cytogenetic analysis. Plant Cell Rep 30, 1415–1425 (2011). https://doi.org/10.1007/s00299-011-1050-x

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  • DOI: https://doi.org/10.1007/s00299-011-1050-x

Keywords

  • Citrus
  • Somatic hybrid
  • Meiotic configuration
  • Intermediate inheritance
  • Molecular analysis