Abstract
Harpin elicits rapid and localized programmed cell death in plants, also known as the hypersensitive response (HR). Here we report that HrpN from Erwinia amylovora led to rapid cell death in maize leaves within 24 h and also induced the expression of systemic acquired resistance genes, such as ZmPR1 and ZmPR5. Surprisingly, the results of DAB staining showed that there was no H2O2 accumulation in maize leaves during the HR process, and semi-quantitative RT-PCR revealed that there was also no difference in the expression of the ZmRboh genes. These results suggest that HrpN-induced cell death may be independent of H2O2 accumulation in maize leaves.
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Acknowledgments
We thank to Professor Han-song Dong for providing HrpN protein. Funding for this research was provided by the Grants from the Nation Natural Science Foundation of China (Nos. 30871457, 31071337) and the State Key Basic Research and Development Plan of China (No. 2009CB118500).
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Communicated by H. Judelson.
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Kong, X., Li, D. Hydrogen peroxide is not involved in HrpN from Erwinia amylovora-induced hypersensitive cell death in maize leaves. Plant Cell Rep 30, 1273–1279 (2011). https://doi.org/10.1007/s00299-011-1038-6
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DOI: https://doi.org/10.1007/s00299-011-1038-6