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Effects of 2-aminoindan-2-phosphonic acid treatment on the accumulation of salidroside and four phenylethanoid glycosides in suspension cell culture of Cistanche deserticola

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Abstract

2-Aminoindan-2-phosphonic acid (AIP), a specific competitive phenylalanine ammonia lyase (PAL) inhibitor was applied to a suspension cell culture of Cistanche deserticola. The effects of AIP treatment on cell growth, PAL activity, contents and yields of total phenolic compound, salidroside and four phenylethanoid glycosides (PheGs) are investigated. The results demonstrated that, 0.5 and 2.0 μM AIP treatments had similar effects on the measurements investigated in this study. AIP treatment resulted in significant decreases in PAL activity, total phenolic compounds content, and PheGs content. Linear regression analysis showed that PAL activity had a high correlation coefficient with the total phenolic compound content and the four PheGs contents. Total PAL activity-time area under curve (AUC) had a high correlation coefficient with the total phenolic compound yield and the yields of five tested compounds in untreated cell samples. In AIP-treated cells, total PAL activity-time AUC retained a high correlation with the total phenolic compound yield and the yields of three tested compounds, echinacoside, acteoside, and tubuloside A, but not salidroside and cistanoside A. The difference could be caused by the different biosynthetic origins of each of the tested compounds. These results demonstrate the important role of PAL in the biosynthesis of PheGs in the suspension cell culture of C. deserticola.

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Acknowledgments

This work was supported by the Dong-A University research fund. We would like to thank Professor Jerry Zon who works at the Institute of Organic Chemistry Biochemistry and Biotechnology, Wrocław University of Technology in Poland for providing us AIP. We also would like to show our appreciation to Dr. Nobuhisa Ezaki at the Yomeishu Seizo Company in Japan for giving us standard compounds cistanoside A, acteoside and tubuloside A for our experiments.

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Authors and Affiliations

  1. College of Life Science and Natural Resources, Dong-A University, Busan, 604-714, Republic of Korea

    Gao Sheng Hu, Yeon Jae Hur, Jai Heon Lee, Young Soo Chung, Young Byung Yi & Doh Hoon Kim

  2. School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016, People’s Republic of China

    Jing Ming Jia

  3. Joint Laboratory of Shenyang Pharmaceutical University and Dong-A University, Shenyang, 110016, People’s Republic of China

    Jing Ming Jia & Doh Hoon Kim

  4. Division of Applied Life Science, Gyeongsang National University, Jinju, 660-701, Republic of Korea

    Dae Jin Yun

  5. Division of Plant Biosciences, Kyungpook National University, Daegu, 702-701, Republic of Korea

    Soon Ki Park

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  1. Gao Sheng Hu
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  2. Yeon Jae Hur
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  3. Jing Ming Jia
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Correspondence to Doh Hoon Kim.

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Communicated by Q. Zhao.

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Hu, G.S., Hur, Y.J., Jia, J.M. et al. Effects of 2-aminoindan-2-phosphonic acid treatment on the accumulation of salidroside and four phenylethanoid glycosides in suspension cell culture of Cistanche deserticola . Plant Cell Rep 30, 665–674 (2011). https://doi.org/10.1007/s00299-010-0997-3

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  • DOI: https://doi.org/10.1007/s00299-010-0997-3

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