Tissue culture-mediated biotechnological intervention in pomegranate: a review

Abstract

The past 30 years have witnessed a series of systematic biotechnological advances made in pomegranate. These encompass optimization and establishment of in vitro culture techniques including micropropagation, somatic embryogenesis, synthetic seed production, plant regeneration via callus-mediated shoot organogenesis, adventitious shoot regeneration, anther culture, tetraploid induction and genetic transformation. This review attempts to provide a comprehensive account on the tissue culture-mediated biotechnological interventions made in pomegranate aimed at complementing conventional programmes for improvement of this nutraceutically important fruit crop.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2

Abbreviations

AC:

Activated charcoal

AS:

Adenine sulfate

AVG:

Aminoethoxyvinylglycine

BAP:

6-Benzylaminopurine

B5 :

Gamborg’s B5 (1968) medium

CH:

Casein hydrolysate

CW:

Coconut water

2,4-D:

2,4-Dichlorophenoxyacetic acid

GA3 :

Gibberellic acid

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

IPA:

Indole-3-propionic acid

Kin:

Kinetin

MS:

Murashige and Skoog (1962) medium

NAA:

Naphthaleneacetic acid

PGRs:

Plant growth regulators

PVP:

Polyvinylpyrrolidone

WPM:

Lloyd and McCown (1980) woody plant medium

ZR:

Zeatin riboside

References

  1. Al-Wasel ASA (1999) In vitro clonal propagation of “Al-Belehi” pomegranate (Punica granatum L.). J King Saud Univ 11:3–14

    Google Scholar 

  2. Amin MN, Islam MN, Azad MAK (1999) Regeneration of plantlets in vitro from the seedling explants of pomegranate (Punica granatum). Plant Tissue Cult 9:53–61

    Google Scholar 

  3. Anonymous (1982) A dictionary of indian raw materials and industrial products. vol VIII (Ph-Re), CSIR Publication, New Delhi, India

  4. Benson EE (2000) In vitro recalcitrance: an introduction. In Vitro Cell Dev Biol Plant 36:141–148

    Article  Google Scholar 

  5. Blazquez MA, Weigel D (2000) Integration of floral inductive signals in Arabidopsis. Nature 889–892

  6. Borner R, Kampmann G, Chandler J, Gleissner R, Wisman E, Apel K, Melzer S (2000) A MADS domain gene involved in the transition to flowering in Arabidopsis. Plant J 24:591–599

    PubMed  Article  CAS  Google Scholar 

  7. Caceres A, Giron LM, Alvarado SR, Torres MF (1987) Screening of antimicrobial activity of plants popularly used in Guatemala for treatment of dermatomucosal diseases. J Ethnopharmacol 20:223–237

    PubMed  Article  CAS  Google Scholar 

  8. Deepika R, Kanwar K (2010) In vitro regeneration of Punica granatum L. plants from different juvenile explants. J Fruit Ornam Plant Res 18:5–22

    CAS  Google Scholar 

  9. El-Agamy SZ, Mostafa RAA, Shaaban MM, El-Mahdy MT (2009) In vitro propagation of Manfalouty and Nab El-gamal pomegranate cultivars. Res J Agric Biol Sci 5:1169–1175

    CAS  Google Scholar 

  10. Ercisli S, Agar G, Orhan E, Yildirim A, Hizarci Y (2007) Interspecific variability of RAPD and fatty acid composition of some pomegranate cultivars (Punica granatum L.) growing in Southern Anatolia Region in Turkey. Biochem Syst Ecol 35:764–769

    Article  CAS  Google Scholar 

  11. Foughat RS, Pandya SB, Ahmad T, Godhani PR (1997) In vitro studies in pomegranate (Punica granatum L.). J Appl Hortic Navsari 3:23–29

    Google Scholar 

  12. Gamborg OL, Miller RA, Ojima KI (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158

    PubMed  Article  CAS  Google Scholar 

  13. Jaidka K, Mehra PN (1986) Morphogenesis in Punica granatum (Pomegranate). Can J Bot 64:1644–1653

    Article  Google Scholar 

  14. Jain SM (2001) Tissue culture-derived variation in crop improvement. Euphytica 118:153–166

    Article  CAS  Google Scholar 

  15. Jimenez VM (2005) Involvement of plant hormones and plant growth regulators on in vitro somatic embryogenesis. Plant Growth Regul 47:91–110

    Article  CAS  Google Scholar 

  16. Julie Jurenka MT (2008) Therapeutic applications of pomegranate (Punica granatum L.): a review. Alt Med Rev 13:128–144

    Google Scholar 

  17. Kantharajah AS, Dewitz I, Jabbari S (1998) Auswirkungen unterschiedlicher Nahrmedien, Wachstums-regulatoren und Explantatquellen auf die in vitro-Vermehrung von Granatapfel (Punica granatum L.) [The effect of media, plant growth regulators and source of explants on in vitro culture of pomegranate (Punica granatum L.). Erwerbsobstbau 40:54–58

    Google Scholar 

  18. Kanwar K, Joseph J, Deepika R (2010) Comparison of in vitro regeneration pathways in Punica granatum L. Plant Cell Tissue Organ Cult 100:199–207

    Article  CAS  Google Scholar 

  19. Krul WR, Myerson J (1980) In vitro propagation of grape. In: Zimmerman RH (ed) Proceedings of the conference on nursery production of fruit plants through tissue culture-application and feasibility. Agric Res Sci Educ Admin, USDA, Beltsville, pp 35–43

    Google Scholar 

  20. Kumar GNM (1990) Pomegranate. In: Nagy S, Shaw PE, Wordowski WF (eds) Fruits of tropical and subtropical origin. Florida Science Source, Inc, USA, pp 328–347

    Google Scholar 

  21. Lad V, Frawley D (1986) The yoga of herbs. Lotus Press, Santa Fe, pp 135–136

    Google Scholar 

  22. Lansky EP, Newman RA (2007) Punica granatum (pomegranate) and its potential for prevention and treatment of inflammation and cancer. J Ethnopharmacol 109:177–206

    PubMed  Article  CAS  Google Scholar 

  23. Lloyd GB, McCown BH (1980) Commercially feasible micropropagation of mountain laurel, Kalmia latifolia by use of shoot tip culture. Proc Plant Prop Soc 30:421–427

    Google Scholar 

  24. Mahishi DM, Muralikrishna A, Shivashankar G, Kulkarni RS (1991) Shoot tip culture method for rapid clonal propagation of pomegranate (Punica granatum L.). In: Prakash J, Pierik RLM (eds) Horticulture-new technologies and applications: proceedings of the international seminar on new frontiers in horticulture. Kluwer Academic Publishers, Dordrecht, pp 215–217

    Google Scholar 

  25. Malik SK, Chaudhury R, Kalia RK (2005) Rapid in vitro multiplication and conservation of Garcinia indica: a tropical medicinal tree species. Sci Hortic 106:539–553

    Article  Google Scholar 

  26. Maroto JV, Miguel A, Lopez-Galarza S, San Bautista A, Pascual B, Alagarda J, Guardiola JL (2005) Parthenocarpic fruit set in triploid watermelon induced by CPPU and 2, 4-D applications. Plant Growth Regul 45:209–213

    Article  CAS  Google Scholar 

  27. Mascarenhas AF, Gupta PK, Kulkarni VM, Mehta U, Iyer RS, Khuspe SS, Jagannathan V (1981) Propagation of trees by tissue culture. In: Rao AN (ed) Proceedings of COSTED symposium on tissue culture of economically important plants. Singapore Institute of Biology, Bangalore, pp 175–179

    Google Scholar 

  28. Mascarenhas AF, Nair S, Iyer RS, Gupta PK (1988) Genetic improvement of fruit crops through tissue culture. In: Genetic manipulation in crops—Proceedings of the international symposium on genetic manipulation in crops, the third international symposium on haploidy; the first international symposium on somatic cell genetics in crops (Beijing)—1984. Cassell Tycooly, pp 41

  29. Miller CO (1965) Evidence for the natural occurrence of zeatin and derivatives compounds from maize which promote cell division. Proc Natl Acad Sci USA 54:1052–1058

    PubMed  Article  CAS  Google Scholar 

  30. Moriguchi T, Omura T, Matsuta N, Kozaki I (1987) In vitro adventitious shoot formation from anthers of pomegranate. Hortic Sci 22:947–948

    Google Scholar 

  31. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  32. Murkute AA, Patil S, Patil BN, Mayakumari MS (2002) Micropropagation in pomegranate, callus induction and differentiation. South Indian Hortic 50:49–55

    Google Scholar 

  33. Murkute AA, Patil S, Mayakumari (2003) Exudation and browning in tissue culture of pomegranate. Agric Sci Digest 23:29–31

  34. Murkute AA, Patil S, Singh SK (2004) In vitro regeneration in pomegranate cv. Ganesh from mature trees. Indian J Hortic 61:206–208

    Google Scholar 

  35. Naik SK, Chand PK (2001) In vitro cloning of a medicinally important fruit tree Punica granatum L. by nodal culture. In: Sahoo S, Ramesh DB, Rao YR, Debata BK, Mishra VN (eds) Conservation and utilization of medicinal and aromatic plants. Allied Publishers Limited, New Delhi, pp 313–322

    Google Scholar 

  36. Naik SK, Chand PK (2003) Silver nitrate and aminoethoxyvinylglycine promote in vitro adventitious shoot regeneration of pomegranate (Punica granatum L.). J Plant Physiol 160:423–430

    PubMed  Article  CAS  Google Scholar 

  37. Naik SK, Chand PK (2006) Nutrient-alginate encapsulation of in vitro nodal segments of pomegranate (Punica granatum L.) for germplasm distribution and exchange. Sci Hortic 108:247–252

    Article  CAS  Google Scholar 

  38. Naik SK, Pattnaik S, Chand PK (1999) In vitro propagation of pomegranate (Punica granatum L. cv. Ganesh) through axillary shoot proliferation from nodal segments of mature tree. Sci Hortic 79:175–183

    Article  CAS  Google Scholar 

  39. Naik SK, Pattnaik S, Chand PK (2000) High frequency axillary shoot proliferation and plant regeneration from cotyledonary nodes of pomegranate (Punica granatum L.). Sci Hortic 85:261–270

    Article  CAS  Google Scholar 

  40. Naqvi SA, Khan MS, Vohora SB (1991) Antibacterial, antifungal, and antihelminthic investigations on Indian medicinal plants. Fitoterapia 62:221–228

    Google Scholar 

  41. Nataraja K, Neelambika GK (1996) Somatic embryogenesis and plantlet from petal cultures of pomegranate, Punica granatum L. Ind J Exp Biol 34:719–721

    CAS  Google Scholar 

  42. Nitsch JP (1969) Experimental androgenesis in Nicotiana. Phytomorphology 19:389–404

    Google Scholar 

  43. Nitsch JP, Nitsch C (1969) Haploid plants from pollen grains. Science 163:85–87

    PubMed  Article  CAS  Google Scholar 

  44. Omura M (1991) Pomegranate (Punica granatum L.). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 16, Trees III. Springer, Berlin, pp 44–61

    Google Scholar 

  45. Omura M, Matsuta T, Moriguchi T, Kozaki I (1987a) Adventitious shoot and plantlet formation from cultured pomegranate leaf explants. Hortic Sci 22:133–134

    CAS  Google Scholar 

  46. Omura M, Matsuta T, Moriguchi T, Kozaki I, Sanada T (1987b) Establishment of tissue culture methods in dwarf pomegranate (Punica granatum L. var. nana) and application for induction of variants. Bull Fruit Tree Res Stn 14:17–44

    Google Scholar 

  47. Omura M, Matsuta N, Moriguchi T, Kozaki I (1990) Suspension culture and plantlet regeneration in dwarf pomegranate (Punica granatum L. var. nana Pers.). Bull Fruit Tree Res Stn 17:19–33

    Google Scholar 

  48. Rai MK, Asthana P, Singh SK, Jaiswal VS, Jaiswal U (2010) Biotechnological advances in guava (Psidium guajava L.): recent developments and prospects for further research. Trees Struct Funct 24:1–12

    CAS  Google Scholar 

  49. Raj Bhansali R (1990) Somatic embryogenesis and regeneration of plantlets in pomegranate. Ann Bot 66:249–253

    Google Scholar 

  50. Rout GR, Mohapatra A, Jain SM (2006) Tissue culture of ornamental pot plant: a critical review on present scenario and future prospects. Biotechnol Adv 24:531–560

    PubMed  Article  CAS  Google Scholar 

  51. Saxena A, Vikram NK (2004) Role of selected Indian plants in management of type 2 diabetes: a review. J Altern Complement Med 10:369–378

    PubMed  Article  Google Scholar 

  52. Shao J, Chen C, Deng X (2003) In vitro induction of tetraploid in pomegranate (Punica granatum). Plant Cell Tissue Organ Cult 75:241–246

    Article  CAS  Google Scholar 

  53. Sharon M, Sinha S (2000) Plant regeneration from cotyledonary node of Punica granatum L. Ind J Plant Physiol 5:344–348

    CAS  Google Scholar 

  54. Sharp WR, Sondahl MR, Caldas LS, Maraffa SB (1980) The physiology of in vitro asexual embryogenesis. Hortic Rev 268–310

  55. Singh SK, Khawale RN (2006) Plantlet regeneration from the nodal segments of pomegranate (Punica granatum L.) cv. Jyoti. In: Kumar A, Roy S (eds) Plant Biotechnology and its applications in tissue culture. IK International Pvt. Ltd., New Delhi, pp 107–113

    Google Scholar 

  56. Sinha S, Sharon M (1997) Somatic embryogenesis and plant regeneration from roots of Punica granatum L. Hortic Sci 32:545–546

    Google Scholar 

  57. Smith PM (1976) Minor crops. In: Simmonds NW (ed) Evolution of crop plants. Longman, New York

    Google Scholar 

  58. Stover E, Mercure EW (2007) The pomegranate: a new look at the fruit of paradise. Hortic Sci 42:1088–1092

    Google Scholar 

  59. Terakami S, Matsuta N, Yamamoto T, Sugaya S, Gemma H, Soejima J (2007) Agrobacterium-mediated transformation of the dwarf pomegranate (Punica granatum L. var. nana). Plant Cell Rep 26:1243–1251

    PubMed  Article  CAS  Google Scholar 

  60. White PR (1943) A hand book of plant tissue culture. Jacques Cattell Press, Lancaster

    Google Scholar 

  61. White PR (1963) The cultivation of animal and plant cells. Ronalds Press, New York

    Google Scholar 

  62. Yang Z, Ludders P (1993) Organogenesis of Punica granatum L. var. nana. Angew Bot 67:151–156

    CAS  Google Scholar 

  63. Zhang B, Stoltz LP (1991) In vitro shoot formation and elongation of dwarf pomegranate. Hortic Sci 26:1084

    Google Scholar 

Download references

Acknowledgments

The authors acknowledge the copyright permission from ‘Elsevier’ for reproducing some photographs of Fig. 2 as follows. Figure 2a: Reprinted from ‘Scientia Horticulturae’, vol. 79, Naik SK, Pattnaik S, Chand PK, In vitro propagation of pomegranate (Punica granatum L. cv. Ganesh) through axillary shoot proliferation from nodal segments of mature tree, pp. 175–183, Copyright (1999), with permission from Elsevier; Fig. 2b: reprinted from ‘Scientia Horticulturae’ vol. 85, Naik SK, Pattnaik S, Chand PK, High frequency axillary shoot proliferation and plant regeneration from cotyledonary nodes of pomegranate (Punica granatum L.), pp. 261–270, Copyright (2000), with permission from Elsevier; Fig. 2h, i and j: Reprinted from ‘Journal of Plant Physiology’, vol. 160, Naik SK, Chand PK, Silver nitrate and aminoethoxyvinylglycine promote in vitro adventitious shoot regeneration of pomegranate (Punica granatum L.), pp. 423–430, Copyright Elsevier (2003), with permission from Global Rights::Elsevier.

Author information

Affiliations

Authors

Corresponding author

Correspondence to Pradeep K. Chand.

Additional information

A contribution to the Special Issue: Plant Biotechnology in Support of the Millennium Development Goals.

Communicated by R. Reski.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Naik, S.K., Chand, P.K. Tissue culture-mediated biotechnological intervention in pomegranate: a review. Plant Cell Rep 30, 707–721 (2011). https://doi.org/10.1007/s00299-010-0969-7

Download citation

Keywords

  • Pomegranate (Punica granatum L.)
  • Micropropagation
  • Somatic embryogenesis
  • Synthetic seed
  • Organogenesis
  • Anther culture
  • Genetic transformation