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Quality evaluation of snow lotus (Saussurea): quantitative chemical analysis and antioxidant activity assessment

Abstract

Snow lotus is commonly used as a medicinal plant and has great pharmacological value. To protect these endangered plants, in vitro propagation and cell cultures have been established in order to meet the growing market demand. The phenolic composition, antioxidant activities, total phenolic content (TPC) and total flavonoid content (TFC) from three most commonly used species, in vitro propagated lines and the cell cultures were investigated to qualify their pharmacological value. Quantitative analysis showed that the phenolics varied greatly among different species and the same species at different habitats. From this it can be inferred that the phenolics were influenced by genetic background and the environmental conditions. Significant correlations were observed between the antioxidant activity and several phenolics/TPC/TFC, suggesting that the phenolics are a major contributor of the antioxidant activity and are important for quality evaluation of snow lotus. Based on the abundance of phenolics, TPC, TFC and antioxidant activity, the order of the quality for wild species would be Saussurea involucrata > Saussurea medusa > Saussurea gossypiphora. For S. medusa, its quality judged by origin would be Shigatse > Lhasa > Nagqu. For in vitro propagated plants, the matured plants could be a reliable substitute for wild plants, and the dynamics of phenolics is critical for quality control of this monocarpic species. We provide the first report of quality comparison between the wild plants and the cell cultures. The advantages of developing cell cultures as alternatives for plants collected from the wild are discussed.

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Abbreviations

UPLC:

Ultra-performance liquid chromatography

HPLC:

High-performance liquid chromatography

ABTS:

2,2′-Azinobis (3-ethylbenzothiazoline-6-sulphonic acid)

TPTZ:

2,4,6-Tripyridyl-S-triazine

Trolox:

6-Hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid

FRAP:

Ferric reducing/antioxidant power

TEAC:

Trolox equivalent antioxidant capacity

TPC:

Total phenolic content

TFC:

Total flavonoid content

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Acknowledgments

Identification of the wild snow lotus plants were done by Dr. Yilin Chen (Institute of Botany, Chinese Academy of Sciences, Beijing, China). Dr. Liansheng Wang (Institute of Botany, Chinese Academy of Sciences, Beijing) is acknowledged for his generous gift of the standards. Financial support was from the National Natural Science Foundation of China (No. 30472158) and the Chinese Academy of Sciences (No. KGCX2–YW–509).

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Correspondence to Qiao Zhao or Dexiu Zhao.

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Communicated by P. Kumar.

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Qiu, J., Xue, X., Chen, F. et al. Quality evaluation of snow lotus (Saussurea): quantitative chemical analysis and antioxidant activity assessment. Plant Cell Rep 29, 1325–1337 (2010). https://doi.org/10.1007/s00299-010-0919-4

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  • DOI: https://doi.org/10.1007/s00299-010-0919-4

Keywords

  • Phenolics
  • Flavonoid
  • Antioxidant activity
  • Snow lotus
  • Saussurea involucrata
  • Saussurea medusa
  • Saussurea gossypiphora
  • UPLC