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Meiotic behavior of pollen mother cells in relation to ploidy level of somatic hybrids between Solanum tuberosum and S. chacoense

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Abstract

Potato somatic hybrids obtained by protoplast fusion between Solanum tuberosum (4x) and Solanum chacoense (2x) were investigated for genome stability and meiotic behavior associated with the pollen viability in order to elucidate the mechanism influencing the fertility of the somatic hybrids. The ploidy level detections conducted in 2004 and 2007 demonstrated that 68 out of 108 somatic hybrids had their ploidy level changed to be uniform and euploidy after successive in vitro subcultures, which mainly occurred in octaploids, aneuploids, and mixoploids, while 74% hexaploids were still stable in their genome dosage in 2007. Different types of abnormal meiotic behavior were observed during the development of pollen mother cells (PMCs) including the formation of univalents, multivalents, laggard chromosomes, and chromosomal bridges, as well as triads and polyads. A higher proportion of abnormal meiosis seemed to be accompanied with a genome dosage higher than the hexaploids expected in this study. A significant positive correlation between defective PMCs and the number of small pollen grains and negative correlation between number of small pollen grains and pollen viability strongly suggested that abnormal meiosis could be a causal factor influencing the fertility of the somatic hybrids. The hexaploids with stable genome dosage and a certain level of fertility will have great potential in a potato breeding program.

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Acknowledgments

The authors are grateful to Prof. Jeff Moorby for critical reading and language editing of this manuscript. We thank the Institute of Vegetables and Flowers of Chinese Academy of Agricultural Sciences for providing the plant materials (3# and 8#). The work was supported by the National 863 Project of China (No. 2006AA100107) and the National Science Foundation of China (No. 30500323).

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Authors and Affiliations

  1. National Center for Vegetable Improvement (Central China), Key Laboratory of Horticultural Plant Biology of Ministry of Education, Potato Engineering and Technology Research Center of Hubei Province, Huazhong Agricultural University, Wuhan, 430070, Hubei, China

    Xianpu Guo, Conghua Xie, Xingkui Cai, Botao Song, Li He & Jun Liu

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  1. Xianpu Guo
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  2. Conghua Xie
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  3. Xingkui Cai
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  4. Botao Song
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  5. Li He
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  6. Jun Liu
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Correspondence to Jun Liu.

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Communicated by Y. Lu.

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Guo, X., Xie, C., Cai, X. et al. Meiotic behavior of pollen mother cells in relation to ploidy level of somatic hybrids between Solanum tuberosum and S. chacoense . Plant Cell Rep 29, 1277–1285 (2010). https://doi.org/10.1007/s00299-010-0914-9

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  • DOI: https://doi.org/10.1007/s00299-010-0914-9

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