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Plant Cell Reports

, Volume 29, Issue 11, pp 1261–1268 | Cite as

Bacterially expressed dsRNA protects maize against SCMV infection

  • Defang Gan
  • Jiao Zhang
  • Haibo Jiang
  • Tong Jiang
  • Suwen Zhu
  • Beijiu Cheng
Original Paper

Abstract

RNA interference (RNAi) is a sequence-specific, posttranscriptional gene silencing (PTGS) process in plants that is mediated by dsRNA homologous to the silenced gene(s). In this study, we report an efficient method to produce dsRNA using a bacterial expression system. Two fragments of the Sugarcane Mosaic Virus (SCMV) CP (coat protein) gene were amplified by RT-PCR, and cloned into the inverted-repeat cloning vector pUCCRNAi. The two recombinant plasmids were transformed individually into E. coli HT115, an RNase-III deficient strain, and dsRNA was induced by isopropyl-β-d-thiogalactopyranoside (IPTG). The crude extracts of E. coli HT115 containing large amounts of dsRNA were applied to plants as a spray and the experiment confirmed a preventative efficacy. Our findings demonstrated that spraying crude dsRNA-containing extracts inhibited SCMV infection, and the dsRNA derived from an upstream region (CP1) was more effective than was dsRNA derived from a downstream region (CP2) of the SCMV CP gene. The results provide a valuable tool for plant viral control using dsRNA and the PTGS approach.

Keywords

Maize Sugarcane Mosaic Virus (SCMV) RNAi dsRNA RNA-mediated virus resistance 

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Defang Gan
    • 1
    • 2
  • Jiao Zhang
    • 2
  • Haibo Jiang
    • 1
  • Tong Jiang
    • 3
  • Suwen Zhu
    • 1
  • Beijiu Cheng
    • 1
  1. 1.Anhui Province Key Laboratory of Crop Biology, School of Life ScienceAnhui Agricultural UniversityHefeiChina
  2. 2.School of HorticultureAnhui Agricultural UniversityHefeiChina
  3. 3.School of Plant ProtectionAnhui Agricultural UniversityHefeiChina

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