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Cell wall alterations in the leaves of fusariosis-resistant and susceptible pineapple cultivars

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Abstract

Fusariosis, caused by the fungus Fusarium subglutinans f. sp. ananas (Syn. F. guttiforme), is one of the main phytosanitary threats to pineapple (Ananas comosus var. comosus). Identification of plant cell responses to pathogens is important in understanding the plant–pathogen relationship and establishing strategies to improve and select resistant cultivars. Studies of the structural properties and phenolic content of cell walls in resistant (Vitoria) and susceptible (Perola) pineapple cultivars, related to resistance to the fungus, were performed. The non-chlorophyll base of physiologically mature leaves was inoculated with a conidia suspension. Analyses were performed post-inoculation by light, atomic force, scanning and transmission electron microscopy, and measurement of cell wall-bound phenolic compounds. Non-inoculated leaves were used as controls to define the constitutive tissue characteristics. Analyses indicated that morphological differences, such as cell wall thickness, cicatrization process and lignification, were related to resistance to the pathogen. Atomic force microscopy indicated a considerable difference in the mechanical properties of the resistant and susceptible cultivars, with more structural integrity, associated with higher levels of cell wall-bound phenolics, found in the resistant cultivar. p-Coumaric and ferulic acids were shown to be the major phenolics bound to the cell walls and were found in higher amounts in the resistant cultivar. Leaves of the resistant cultivar had reduced fungal penetration and a faster and more effective cicatrization response compared to the susceptible cultivar.

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Acknowledgments

The authors thank Giovana A. de Moraes and Beatriz F. Ribeiro for technical assistance with the electron microscopy and Paulo Moscon for technical assistance with the atomic force microscopy. This work was supported by Financiadora de Estudos e Projetos (FINEP), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Banco do Nordeste do Brasil (BNB) and Fundação de Amparo à Pesquisa do Estado do Espírito Santo (FAPES).

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Authors and Affiliations

  1. Núcleo de Biotecnologia, Universidade Federal do Espírito Santo, Vitoria, ES, 29040-090, Brazil

    Glória Maria de Farias Viégas Aquije, Poliana Belisário Zorzal, David Shaun Buss, Patricia Machado Bueno Fernandes & Antonio Alberto Ribeiro Fernandes

  2. Instituto Capixaba de Pesquisa, Assistência Técnica e Extensão Rural, Incaper, Vitoria, ES, 29052-010, Brazil

    José Aires Ventura

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  1. Glória Maria de Farias Viégas Aquije
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  2. Poliana Belisário Zorzal
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  3. David Shaun Buss
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  4. José Aires Ventura
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  5. Patricia Machado Bueno Fernandes
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  6. Antonio Alberto Ribeiro Fernandes
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Correspondence to Glória Maria de Farias Viégas Aquije.

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Communicated by P. Puigdomenech.

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de Farias Viégas Aquije, G.M., Zorzal, P.B., Buss, D.S. et al. Cell wall alterations in the leaves of fusariosis-resistant and susceptible pineapple cultivars. Plant Cell Rep 29, 1109–1117 (2010). https://doi.org/10.1007/s00299-010-0894-9

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  • DOI: https://doi.org/10.1007/s00299-010-0894-9

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